human RPE65 (NM_000329) lentivirus particles

Name: human RPE65 (NM_000329) lentivirus particles
SKU: LV24270L
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : LV24270L

Storage : -80℃ Shipping : Frozen on dry ice

Titer: Size:

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Virus Particles Information

Quality Control

Gene Information

Cat. No.	LV24270L
Description	Lentiviral particles of retinal pigment epithelium-specific protein 65kDa (RPE65).
Product Type	Premade Lentivirus Particles
Gene	RPE65
Species	Human
Insert Size	1602 bp
Titer	Varies lot by lot, for example, ≥110^7 TU/mL, ≥110^8 TU/mL, ≥1*10^9 TU/mL etc.
Size	Varies lot by lot, for example, 100 ul, 500 ul, 1 mL etc.
Storage	Store at -80℃. Avoid multiple freeze/thaw cycles.
Shipping	Frozen on dry ice

Summary	Creative Biogene ensures high-quality lentivirus particles by optimizing and standardizing production protocols and performing stringent quality control (QC). The specific QC experiments performed vary between lentivirus particle lots.
Mycoplasma	Creative Biogene routinely tests for mycoplasma contamination using a mycoplasma detection kit. Cell lines are maintained for approximately 20 passages before being discarded and replaced with a new vial of early passage cells. Approximately 2 weeks after thawing, cell culture supernatants are tested for mycoplasma contamination. Creative Biogene ensures that lentiviral products are free of mycoplasma contamination.
Purity	Creative Biogene evaluates the level of impurities, such as residual host cell DNA or proteins, in prepared lentiviral vectors to ensure they meet quality standards.
Sterility	The lentiviral samples were inoculated into cell culture medium for about 5 days and the growth of bacteria and fungi was tested. Creative Biogene ensures that the lentiviral products are free of microbial contamination.
Transducibility	Upon requirement, Creative Biogene can perform in vitro or in vivo transduction assays to evaluate the ability of lentivirus to deliver genetic material into target cells, and assess gene expression and functional activities.
Proviral Identity Confirmation	All Creative Biogene lentiviral vectors are confirmed to have correctly integrated provirus using PCR. This test involves transducing cells with serial dilutions of the lentiviral vector, harvesting the cells a few days later, and isolating genomic DNA. This DNA is then used as a template to amplify a portion of the expected lentiviral insert.

Gene Name	RPE65 retinal pigment epithelium-specific protein 65kDa [ Homo sapiens ]
Gene Symbol	RPE65
Synonyms	LCA2; RP20; rd12; mRPE65; sRPE65
Gene Description	retinal pigment epithelium-specific protein 65kDa
Gene ID	6121
Uni Prot ID	Q16518
m RNA Refseq	NM_000329.2
Protein Refseq	NP_000320.1
Chromosome Location	1p31
Pathway	Retinol metabolism, organism-specific biosystem; Retinol metabolism, conserved biosystem; Visual signal transduction: Cones, organism-specific biosystem; Visual signal transduction: Rods, organism-specific biosystem; Vitamin A and carotenoid metabolism, organism-specific biosystem; the visual cycle, organism-specific biosystem; the visual cycle I (vertebrates), conserved biosystem;
MIM	180069

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