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human REV3L (NM_002912) lentivirus particles

For research use only. Not intended for any clinical use.

Cat. No. :   LV23833L

Storage :   -80℃ Shipping :   Frozen on dry ice

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Virus Particles Information

Quality Control

Gene Information

Cat. No. LV23833L
Description Lentiviral particles of REV3-like, catalytic subunit of DNA polymerase zeta (yeast) (REV3L).
Product Type Premade Lentivirus Particles
Gene REV3L
Species Human
Insert Size 9393 bp
Titer Varies lot by lot, for example, ≥1*10^7 TU/mL, ≥1*10^8 TU/mL, ≥1*10^9 TU/mL etc.
Size Varies lot by lot, for example, 100 ul, 500 ul, 1 mL etc.
Storage Store at -80℃. Avoid multiple freeze/thaw cycles.
Shipping Frozen on dry ice
Summary Creative Biogene ensures high-quality lentivirus particles by optimizing and standardizing production protocols and performing stringent quality control (QC). The specific QC experiments performed vary between lentivirus particle lots.
Mycoplasma Creative Biogene routinely tests for mycoplasma contamination using a mycoplasma detection kit. Cell lines are maintained for approximately 20 passages before being discarded and replaced with a new vial of early passage cells. Approximately 2 weeks after thawing, cell culture supernatants are tested for mycoplasma contamination. Creative Biogene ensures that lentiviral products are free of mycoplasma contamination.
Purity Creative Biogene evaluates the level of impurities, such as residual host cell DNA or proteins, in prepared lentiviral vectors to ensure they meet quality standards.
Sterility The lentiviral samples were inoculated into cell culture medium for about 5 days and the growth of bacteria and fungi was tested. Creative Biogene ensures that the lentiviral products are free of microbial contamination.
Transducibility Upon requirement, Creative Biogene can perform in vitro or in vivo transduction assays to evaluate the ability of lentivirus to deliver genetic material into target cells, and assess gene expression and functional activities.
Proviral Identity Confirmation All Creative Biogene lentiviral vectors are confirmed to have correctly integrated provirus using PCR. This test involves transducing cells with serial dilutions of the lentiviral vector, harvesting the cells a few days later, and isolating genomic DNA. This DNA is then used as a template to amplify a portion of the expected lentiviral insert.
Gene Name REV3L REV3-like, polymerase (DNA directed), zeta, catalytic subunit [ Homo sapiens ]
Gene Symbol REV3L
Synonyms POLZ; REV3
Gene Description REV3-like, catalytic subunit of DNA polymerase zeta (yeast)
GeneID 5980
Uni ProtID O60673
mRNA Refseq NM_002912.3
Protein Refseq NP_002903.3
Chromosome Location 6q21
Pathway DNA Damage Bypass, organism-specific biosystem; DNA Repair, organism-specific biosystem; DNA polymerase zeta complex, organism-specific biosystem; DNA polymerase zeta complex, conserved biosystem; Fanconi anemia pathway, organism-specific biosystem; Fanconi anemia pathway, conserved biosystem; Translesion synthesis by DNA polymerases bypassing lesion on DNA template, organism-specific biosystem;
MIM 602776
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