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human POLR3E (NM_018119) lentivirus particles

For research use only. Not intended for any clinical use.

Cat. No. :   LV22230L

Storage :   -80℃ Shipping :   Frozen on dry ice

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Virus Particles Information

Quality Control

Gene Information

Cat. No. LV22230L
Description Lentiviral particles of polymerase (RNA) III (DNA directed) polypeptide E (80kD) (POLR3E).
Product Type Premade Lentivirus Particles
Gene POLR3E
Species Human
Insert Size 2127 bp
Titer Varies lot by lot, for example, ≥1*10^7 TU/mL, ≥1*10^8 TU/mL, ≥1*10^9 TU/mL etc.
Size Varies lot by lot, for example, 100 ul, 500 ul, 1 mL etc.
Storage Store at -80℃. Avoid multiple freeze/thaw cycles.
Shipping Frozen on dry ice
Summary Creative Biogene ensures high-quality lentivirus particles by optimizing and standardizing production protocols and performing stringent quality control (QC). The specific QC experiments performed vary between lentivirus particle lots.
Mycoplasma Creative Biogene routinely tests for mycoplasma contamination using a mycoplasma detection kit. Cell lines are maintained for approximately 20 passages before being discarded and replaced with a new vial of early passage cells. Approximately 2 weeks after thawing, cell culture supernatants are tested for mycoplasma contamination. Creative Biogene ensures that lentiviral products are free of mycoplasma contamination.
Purity Creative Biogene evaluates the level of impurities, such as residual host cell DNA or proteins, in prepared lentiviral vectors to ensure they meet quality standards.
Sterility The lentiviral samples were inoculated into cell culture medium for about 5 days and the growth of bacteria and fungi was tested. Creative Biogene ensures that the lentiviral products are free of microbial contamination.
Transducibility Upon requirement, Creative Biogene can perform in vitro or in vivo transduction assays to evaluate the ability of lentivirus to deliver genetic material into target cells, and assess gene expression and functional activities.
Proviral Identity Confirmation All Creative Biogene lentiviral vectors are confirmed to have correctly integrated provirus using PCR. This test involves transducing cells with serial dilutions of the lentiviral vector, harvesting the cells a few days later, and isolating genomic DNA. This DNA is then used as a template to amplify a portion of the expected lentiviral insert.
Gene Name POLR3E polymerase (RNA) III (DNA directed) polypeptide E (80kD) [ Homo sapiens ]
Gene Symbol POLR3E
Synonyms POLR3E; polymerase (RNA) III (DNA directed) polypeptide E (80kD); DNA-directed RNA polymerase III subunit RPC5; FLJ10509; RPC5; SIN; RNA polymerase III subunit C5; RNA polymerase III 80 kDa subunit RPC5; DNA-directed RNA polymerase III 80 kDa polypeptide;
GeneID 55718
Uni ProtID Q9NVU0
mRNA Refseq BC000285
Chromosome Location 16p12
Function DNA-directed RNA polymerase activity;
Pathway Cytosolic DNA-sensing pathway, organism-specific biosystem; Cytosolic DNA-sensing pathway, conserved biosystem; Eukaryotic Transcription Initiation, organism-specific biosystem; Gene Expression, organism-specific biosystem; Purine metabolism, organism-specific biosystem; Purine metabolism, conserved biosystem; Pyrimidine metabolism, organism-specific biosystem;
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