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human POLD1 (NM_002691) lentivirus particles

For research use only. Not intended for any clinical use.

Cat. No. :   LV22180L

Storage :   -80℃ Shipping :   Frozen on dry ice

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Virus Particles Information

Quality Control

Gene Information

Cat. No. LV22180L
Description Lentiviral particles of polymerase (DNA directed), delta 1, catalytic subunit 125kDa (POLD1).
Product Type Premade Lentivirus Particles
Gene POLD1
Species Human
Insert Size 3324 bp
Titer Varies lot by lot, for example, ≥1*10^7 TU/mL, ≥1*10^8 TU/mL, ≥1*10^9 TU/mL etc.
Size Varies lot by lot, for example, 100 ul, 500 ul, 1 mL etc.
Storage Store at -80℃. Avoid multiple freeze/thaw cycles.
Shipping Frozen on dry ice
Summary Creative Biogene ensures high-quality lentivirus particles by optimizing and standardizing production protocols and performing stringent quality control (QC). The specific QC experiments performed vary between lentivirus particle lots.
Mycoplasma Creative Biogene routinely tests for mycoplasma contamination using a mycoplasma detection kit. Cell lines are maintained for approximately 20 passages before being discarded and replaced with a new vial of early passage cells. Approximately 2 weeks after thawing, cell culture supernatants are tested for mycoplasma contamination. Creative Biogene ensures that lentiviral products are free of mycoplasma contamination.
Purity Creative Biogene evaluates the level of impurities, such as residual host cell DNA or proteins, in prepared lentiviral vectors to ensure they meet quality standards.
Sterility The lentiviral samples were inoculated into cell culture medium for about 5 days and the growth of bacteria and fungi was tested. Creative Biogene ensures that the lentiviral products are free of microbial contamination.
Transducibility Upon requirement, Creative Biogene can perform in vitro or in vivo transduction assays to evaluate the ability of lentivirus to deliver genetic material into target cells, and assess gene expression and functional activities.
Proviral Identity Confirmation All Creative Biogene lentiviral vectors are confirmed to have correctly integrated provirus using PCR. This test involves transducing cells with serial dilutions of the lentiviral vector, harvesting the cells a few days later, and isolating genomic DNA. This DNA is then used as a template to amplify a portion of the expected lentiviral insert.
Gene Name POLD1 polymerase (DNA directed), delta 1, catalytic subunit 125kDa [ Homo sapiens ]
Gene Symbol POLD1
Synonyms POLD1; polymerase (DNA directed), delta 1, catalytic subunit 125kDa; POLD, polymerase (DNA directed), delta 1, catalytic subunit (125kD); DNA polymerase delta catalytic subunit; CDC2; CDC2 homolog (S. cerevisiae); CDC2 homolog; DNA polymerase subunit delta p125; POLD;
Gene ID 5424
Uni Prot ID P28340
m RNA Refseq BC008800
Chromosome Location 19q13.3
Function 3-5-exodeoxyribonuclease activity; DNA binding; DNA-directed DNA polymerase activity; chromatin binding; hydrolase activity; metal ion binding; nucleotide binding; nucleotidyltransferase activity; protein binding; transferase activity;
Pathway Base Excision Repair, organism-specific biosystem; Base excision repair, organism-specific biosystem; Base excision repair, conserved biosystem; Cell Cycle, organism-specific biosystem; Cell Cycle, Mitotic, organism-specific biosystem; Chromosome Maintenance, organism-specific biosystem; DNA Repair, organism-specific biosystem;
MIM 174761
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