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human PDHX (NM_003477) lentivirus particles

For research use only. Not intended for any clinical use.

Cat. No. :   LV21392L

Storage :   -80℃ Shipping :   Frozen on dry ice

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Virus Particles Information

Quality Control

Gene Information

Cat. No. LV21392L
Description Lentiviral particles of pyruvate dehydrogenase complex, component X (PDHX), nuclear gene encoding mitochondrial protein, transcript variant 1.
Product Type Premade Lentivirus Particles
Gene PDHX
Species Human
Insert Size 1506 bp
Titer Varies lot by lot, for example, ≥1*10^7 TU/mL, ≥1*10^8 TU/mL, ≥1*10^9 TU/mL etc.
Size Varies lot by lot, for example, 100 ul, 500 ul, 1 mL etc.
Storage Store at -80℃. Avoid multiple freeze/thaw cycles.
Shipping Frozen on dry ice
Summary Creative Biogene ensures high-quality lentivirus particles by optimizing and standardizing production protocols and performing stringent quality control (QC). The specific QC experiments performed vary between lentivirus particle lots.
Mycoplasma Creative Biogene routinely tests for mycoplasma contamination using a mycoplasma detection kit. Cell lines are maintained for approximately 20 passages before being discarded and replaced with a new vial of early passage cells. Approximately 2 weeks after thawing, cell culture supernatants are tested for mycoplasma contamination. Creative Biogene ensures that lentiviral products are free of mycoplasma contamination.
Purity Creative Biogene evaluates the level of impurities, such as residual host cell DNA or proteins, in prepared lentiviral vectors to ensure they meet quality standards.
Sterility The lentiviral samples were inoculated into cell culture medium for about 5 days and the growth of bacteria and fungi was tested. Creative Biogene ensures that the lentiviral products are free of microbial contamination.
Transducibility Upon requirement, Creative Biogene can perform in vitro or in vivo transduction assays to evaluate the ability of lentivirus to deliver genetic material into target cells, and assess gene expression and functional activities.
Proviral Identity Confirmation All Creative Biogene lentiviral vectors are confirmed to have correctly integrated provirus using PCR. This test involves transducing cells with serial dilutions of the lentiviral vector, harvesting the cells a few days later, and isolating genomic DNA. This DNA is then used as a template to amplify a portion of the expected lentiviral insert.
Gene Name PDHX pyruvate dehydrogenase complex, component X [ Homo sapiens ]
Gene Symbol PDHX
Synonyms PDHX; pyruvate dehydrogenase complex, component X; pyruvate dehydrogenase protein X component, mitochondrial; DLDBP; E3BP; OPDX; PDX1; proX; pyruvate dehydrogenase complex, E3-binding protein subunit; lipoyl-containing pyruvate dehydrogenase complex component X; pyruvate dehydrogenase complex, lipoyl-containing component X; dihydrolipoamide dehydrogenase-binding protein of pyruvate dehydrogenase complex;
Gene ID 8050
Uni Prot ID O00330
m RNA Refseq BC010389
Chromosome Location 11p13
Function transferase activity, transferring acyl groups;
Pathway Glycolysis and Gluconeogenesis, organism-specific biosystem; Metabolic pathways, organism-specific biosystem; Metabolism, organism-specific biosystem; Pyruvate metabolism, organism-specific biosystem; Pyruvate metabolism and Citric Acid (TCA) cycle, organism-specific biosystem; Pyruvate oxidation, pyruvate => acetyl-CoA, organism-specific biosystem;
MIM 608769
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