human NOS3 (NM_001160110) lentivirus particles

Name: human NOS3 (NM_001160110) lentivirus particles
SKU: LV19728L
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : LV19728L

Storage : -80℃ Shipping : Frozen on dry ice

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Virus Particles Information

Quality Control

Gene Information

Cat. No.	LV19728L
Description	Lentiviral particles of nitric oxide synthase 3 (endothelial cell) (NOS3), transcript variant 3.
Product Type	Premade Lentivirus Particles
Gene	NOS3
Species	Human
Insert Size	1845 bp
Titer	Varies lot by lot, for example, ≥110^7 TU/mL, ≥110^8 TU/mL, ≥1*10^9 TU/mL etc.
Size	Varies lot by lot, for example, 100 ul, 500 ul, 1 mL etc.
Storage	Store at -80℃. Avoid multiple freeze/thaw cycles.
Shipping	Frozen on dry ice

Summary	Creative Biogene ensures high-quality lentivirus particles by optimizing and standardizing production protocols and performing stringent quality control (QC). The specific QC experiments performed vary between lentivirus particle lots.
Mycoplasma	Creative Biogene routinely tests for mycoplasma contamination using a mycoplasma detection kit. Cell lines are maintained for approximately 20 passages before being discarded and replaced with a new vial of early passage cells. Approximately 2 weeks after thawing, cell culture supernatants are tested for mycoplasma contamination. Creative Biogene ensures that lentiviral products are free of mycoplasma contamination.
Purity	Creative Biogene evaluates the level of impurities, such as residual host cell DNA or proteins, in prepared lentiviral vectors to ensure they meet quality standards.
Sterility	The lentiviral samples were inoculated into cell culture medium for about 5 days and the growth of bacteria and fungi was tested. Creative Biogene ensures that the lentiviral products are free of microbial contamination.
Transducibility	Upon requirement, Creative Biogene can perform in vitro or in vivo transduction assays to evaluate the ability of lentivirus to deliver genetic material into target cells, and assess gene expression and functional activities.
Proviral Identity Confirmation	All Creative Biogene lentiviral vectors are confirmed to have correctly integrated provirus using PCR. This test involves transducing cells with serial dilutions of the lentiviral vector, harvesting the cells a few days later, and isolating genomic DNA. This DNA is then used as a template to amplify a portion of the expected lentiviral insert.

Gene Name	NOS3 nitric oxide synthase 3 (endothelial cell) [ Homo sapiens ]
Gene Symbol	NOS3
Synonyms	NOS3; nitric oxide synthase 3 (endothelial cell); nitric oxide synthase, endothelial; ECNOS; endothelial nitric oxide synthase; eNOS; cNOS; EC-NOS; NOSIII; NOS type III; endothelial NOS; constitutive NOS;
Gene ID	4846
Uni Prot ID	P29474
m RNA Refseq	BC069465
Chromosome Location	7q36
Function	FMN binding; NADP binding; actin monomer binding; arginine binding; cadmium ion binding; calmodulin binding; flavin adenine dinucleotide binding; heme binding; metal ion binding; nitric-oxide synthase activity; oxidoreductase activity; protein binding; tetrahydrobiopterin binding;
Pathway	ACE Inhibitor Pathway, organism-specific biosystem; Angiopoietin receptor Tie2-mediated signaling, organism-specific biosystem; Arginine and proline metabolism, organism-specific biosystem; Arginine and proline metabolism, conserved biosystem; Calcium signaling pathway, organism-specific biosystem; Calcium signaling pathway, conserved biosystem; Hemostasis, organism-specific biosystem;

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