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human NNT (NM_012343) lentivirus particles

For research use only. Not intended for any clinical use.

Cat. No. :   LV19679L

Storage :   -80℃ Shipping :   Frozen on dry ice

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Virus Particles Information

Quality Control

Gene Information

Cat. No. LV19679L
Description Lentiviral particles of nicotinamide nucleotide transhydrogenase (NNT), nuclear gene encoding mitochondrial protein, transcript variant 1.
Product Type Premade Lentivirus Particles
Gene NNT
Species Human
Insert Size 3261 bp
Titer Varies lot by lot, for example, ≥1*10^7 TU/mL, ≥1*10^8 TU/mL, ≥1*10^9 TU/mL etc.
Size Varies lot by lot, for example, 100 ul, 500 ul, 1 mL etc.
Storage Store at -80℃. Avoid multiple freeze/thaw cycles.
Shipping Frozen on dry ice
Summary Creative Biogene ensures high-quality lentivirus particles by optimizing and standardizing production protocols and performing stringent quality control (QC). The specific QC experiments performed vary between lentivirus particle lots.
Mycoplasma Creative Biogene routinely tests for mycoplasma contamination using a mycoplasma detection kit. Cell lines are maintained for approximately 20 passages before being discarded and replaced with a new vial of early passage cells. Approximately 2 weeks after thawing, cell culture supernatants are tested for mycoplasma contamination. Creative Biogene ensures that lentiviral products are free of mycoplasma contamination.
Purity Creative Biogene evaluates the level of impurities, such as residual host cell DNA or proteins, in prepared lentiviral vectors to ensure they meet quality standards.
Sterility The lentiviral samples were inoculated into cell culture medium for about 5 days and the growth of bacteria and fungi was tested. Creative Biogene ensures that the lentiviral products are free of microbial contamination.
Transducibility Upon requirement, Creative Biogene can perform in vitro or in vivo transduction assays to evaluate the ability of lentivirus to deliver genetic material into target cells, and assess gene expression and functional activities.
Proviral Identity Confirmation All Creative Biogene lentiviral vectors are confirmed to have correctly integrated provirus using PCR. This test involves transducing cells with serial dilutions of the lentiviral vector, harvesting the cells a few days later, and isolating genomic DNA. This DNA is then used as a template to amplify a portion of the expected lentiviral insert.
Gene Name NNT nicotinamide nucleotide transhydrogenase [ Homo sapiens ]
Gene Symbol NNT
Synonyms GCCD4
Gene Description nicotinamide nucleotide transhydrogenase
Gene ID 23530
Uni Prot ID Q13423
m RNA Refseq NM_012343.3
Protein Refseq NP_036475.3
Chromosome Location 5p12
Function NAD binding; NAD(P)+ transhydrogenase (AB-specific) activity; NAD(P)+ transhydrogenase (B-specific) activity; NAD(P)+ transhydrogenase activity; NADP binding;
Pathway Citric acid cycle (TCA cycle), organism-specific biosystem; Metabolism, organism-specific biosystem; NAD phosphorylation and dephosphorylation, organism-specific biosystem; Nicotinate and nicotinamide metabolism, organism-specific biosystem; Nicotinate and nicotinamide metabolism, conserved biosystem; Pyruvate metabolism and Citric Acid (TCA) cycle, organism-specific biosystem; The citric acid (TCA) cycle and respiratory electron transport, organism-specific biosystem;
MIM 607878
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