human GTF2F1 (NM_002096) lentivirus particles

Name: human GTF2F1 (NM_002096) lentivirus particles
SKU: LV14252L
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : LV14252L

Storage : -80℃ Shipping : Frozen on dry ice

Titer: Size:

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Virus Particles Information

Quality Control

Gene Information

Cat. No.	LV14252L
Description	Lentiviral particles of general transcription factor IIF, polypeptide 1, 74kDa (GTF2F1).
Product Type	Premade Lentivirus Particles
Gene	GTF2F1
Species	Human
Insert Size	1554 bp
Titer	Varies lot by lot, for example, ≥110^7 TU/mL, ≥110^8 TU/mL, ≥1*10^9 TU/mL etc.
Size	Varies lot by lot, for example, 100 ul, 500 ul, 1 mL etc.
Storage	Store at -80℃. Avoid multiple freeze/thaw cycles.
Shipping	Frozen on dry ice

Summary	Creative Biogene ensures high-quality lentivirus particles by optimizing and standardizing production protocols and performing stringent quality control (QC). The specific QC experiments performed vary between lentivirus particle lots.
Mycoplasma	Creative Biogene routinely tests for mycoplasma contamination using a mycoplasma detection kit. Cell lines are maintained for approximately 20 passages before being discarded and replaced with a new vial of early passage cells. Approximately 2 weeks after thawing, cell culture supernatants are tested for mycoplasma contamination. Creative Biogene ensures that lentiviral products are free of mycoplasma contamination.
Purity	Creative Biogene evaluates the level of impurities, such as residual host cell DNA or proteins, in prepared lentiviral vectors to ensure they meet quality standards.
Sterility	The lentiviral samples were inoculated into cell culture medium for about 5 days and the growth of bacteria and fungi was tested. Creative Biogene ensures that the lentiviral products are free of microbial contamination.
Transducibility	Upon requirement, Creative Biogene can perform in vitro or in vivo transduction assays to evaluate the ability of lentivirus to deliver genetic material into target cells, and assess gene expression and functional activities.
Proviral Identity Confirmation	All Creative Biogene lentiviral vectors are confirmed to have correctly integrated provirus using PCR. This test involves transducing cells with serial dilutions of the lentiviral vector, harvesting the cells a few days later, and isolating genomic DNA. This DNA is then used as a template to amplify a portion of the expected lentiviral insert.

Gene Name	GTF2F1 general transcription factor IIF, polypeptide 1, 74kDa [ Homo sapiens ]
Gene Symbol	GTF2F1
Synonyms	BTF4; RAP74; TF2F1; TFIIF
Gene Description	general transcription factor IIF, polypeptide 1, 74kDa
GeneID	2962
Uni ProtID	P35269
mRNA Refseq	NM_002096.2
Protein Refseq	NP_002087.2
Chromosome Location	19p13.3
Function	DNA binding; catalytic activity; phosphatase activator activity; protein binding; transcription coactivator activity; transcription factor binding;
Pathway	Abortive elongation of HIV-1 transcript in the absence of Tat, organism-specific biosystem; Androgen Receptor Signaling Pathway, organism-specific biosystem; Basal transcription factors, organism-specific biosystem; Basal transcription factors, conserved biosystem; Disease, organism-specific biosystem; Formation of HIV-1 elongation complex containing HIV-1 Tat, organism-specific biosystem; Formation of HIV-1 elongation complex in the absence of HIV-1 Tat, organism-specific biosystem;
MIM	189968

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