Transfected Stable Cell Lines
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Cat. No. : LV10966L
Storage : -80℃ Shipping : Frozen on dry ice
Titer: Size:
| Cat. No. | LV10966L |
| Description | Lentiviral particles of DnaJ (Hsp40) homolog, subfamily C, member 10 (DNAJC10). |
| Product Type | Premade Lentivirus Particles |
| Gene | DNAJC10 |
| Species | Human |
| Insert Size | 2382 bp |
| Titer | Varies lot by lot, for example, ≥1*10^7 TU/mL, ≥1*10^8 TU/mL, ≥1*10^9 TU/mL etc. |
| Size | Varies lot by lot, for example, 100 ul, 500 ul, 1 mL etc. |
| Storage | Store at -80℃. Avoid multiple freeze/thaw cycles. |
| Shipping | Frozen on dry ice |
| Summary | Creative Biogene ensures high-quality lentivirus particles by optimizing and standardizing production protocols and performing stringent quality control (QC). The specific QC experiments performed vary between lentivirus particle lots. |
| Mycoplasma | Creative Biogene routinely tests for mycoplasma contamination using a mycoplasma detection kit. Cell lines are maintained for approximately 20 passages before being discarded and replaced with a new vial of early passage cells. Approximately 2 weeks after thawing, cell culture supernatants are tested for mycoplasma contamination. Creative Biogene ensures that lentiviral products are free of mycoplasma contamination. |
| Purity | Creative Biogene evaluates the level of impurities, such as residual host cell DNA or proteins, in prepared lentiviral vectors to ensure they meet quality standards. |
| Sterility | The lentiviral samples were inoculated into cell culture medium for about 5 days and the growth of bacteria and fungi was tested. Creative Biogene ensures that the lentiviral products are free of microbial contamination. |
| Transducibility | Upon requirement, Creative Biogene can perform in vitro or in vivo transduction assays to evaluate the ability of lentivirus to deliver genetic material into target cells, and assess gene expression and functional activities. |
| Proviral Identity Confirmation | All Creative Biogene lentiviral vectors are confirmed to have correctly integrated provirus using PCR. This test involves transducing cells with serial dilutions of the lentiviral vector, harvesting the cells a few days later, and isolating genomic DNA. This DNA is then used as a template to amplify a portion of the expected lentiviral insert. |
| Gene Name | DNAJC10 DnaJ (Hsp40) homolog, subfamily C, member 10 [ Homo sapiens ] |
| Gene Symbol | DNAJC10 |
| Synonyms | DNAJC10; DnaJ (Hsp40) homolog, subfamily C, member 10; dnaJ homolog subfamily C member 10; ERdj5; PDIA19; protein disulfide isomerase family A; member 19; MTHr; macrothioredoxin; ER-resident protein ERdj5; protein disulfide isomerase family A, member 19; J-domain-containing protein disulfide isomerase-like protein; JPDI; MGC104194; DKFZp434J1813; |
| Gene ID | 54431 |
| Uni Prot ID | Q8IXB1 |
| m RNA Refseq | BC034713 |
| Chromosome Location | 2q32.1 |
| Function | ATPase activator activity; ATPase binding; chaperone binding; disulfide oxidoreductase activity; electron carrier activity; heat shock protein binding; misfolded protein binding; protein binding; protein disulfide oxidoreductase activity; unfolded protein binding; |
| Pathway | Protein processing in endoplasmic reticulum, organism-specific biosystem; Protein processing in endoplasmic reticulum, conserved biosystem; |
| MIM | 607987 |
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