human DFFB (NM_004402) lentivirus particles

Name: human DFFB (NM_004402) lentivirus particles
SKU: LV10630L
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : LV10630L

Storage : -80℃ Shipping : Frozen on dry ice

Titer: Size:

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Virus Particles Information

Quality Control

Gene Information

Cat. No.	LV10630L
Description	Lentiviral particles of DNA fragmentation factor, 40kDa, beta polypeptide (caspase-activated DNase) (DFFB).
Product Type	Premade Lentivirus Particles
Gene	DFFB
Species	Human
Insert Size	1017 bp
Titer	Varies lot by lot, for example, ≥110^7 TU/mL, ≥110^8 TU/mL, ≥1*10^9 TU/mL etc.
Size	Varies lot by lot, for example, 100 ul, 500 ul, 1 mL etc.
Storage	Store at -80℃. Avoid multiple freeze/thaw cycles.
Shipping	Frozen on dry ice

Summary	Creative Biogene ensures high-quality lentivirus particles by optimizing and standardizing production protocols and performing stringent quality control (QC). The specific QC experiments performed vary between lentivirus particle lots.
Mycoplasma	Creative Biogene routinely tests for mycoplasma contamination using a mycoplasma detection kit. Cell lines are maintained for approximately 20 passages before being discarded and replaced with a new vial of early passage cells. Approximately 2 weeks after thawing, cell culture supernatants are tested for mycoplasma contamination. Creative Biogene ensures that lentiviral products are free of mycoplasma contamination.
Purity	Creative Biogene evaluates the level of impurities, such as residual host cell DNA or proteins, in prepared lentiviral vectors to ensure they meet quality standards.
Sterility	The lentiviral samples were inoculated into cell culture medium for about 5 days and the growth of bacteria and fungi was tested. Creative Biogene ensures that the lentiviral products are free of microbial contamination.
Transducibility	Upon requirement, Creative Biogene can perform in vitro or in vivo transduction assays to evaluate the ability of lentivirus to deliver genetic material into target cells, and assess gene expression and functional activities.
Proviral Identity Confirmation	All Creative Biogene lentiviral vectors are confirmed to have correctly integrated provirus using PCR. This test involves transducing cells with serial dilutions of the lentiviral vector, harvesting the cells a few days later, and isolating genomic DNA. This DNA is then used as a template to amplify a portion of the expected lentiviral insert.

Gene Name	DFFB DNA fragmentation factor, 40kDa, beta polypeptide (caspase-activated DNase) [ Homo sapiens ]
Gene Symbol	DFFB
Synonyms	CAD; CPAN; DFF2; DFF40; DFF-40
Gene Description	DNA fragmentation factor, 40kDa, beta polypeptide (caspase-activated DNase)
Gene ID	1677
Uni Prot ID	O76075
m RNA Refseq	NM_004402.2
Protein Refseq	NP_004393.1
Chromosome Location	1p36.3
Function	DNA binding; deoxyribonuclease activity; enzyme binding; nicotinate phosphoribosyltransferase activity;
Pathway	Activation of DNA fragmentation factor, organism-specific biosystem; Apoptosis, organism-specific biosystem; Apoptosis, organism-specific biosystem; Apoptosis, conserved biosystem; Apoptosis, organism-specific biosystem; Apoptosis Modulation and Signaling, organism-specific biosystem; Apoptosis induced DNA fragmentation, organism-specific biosystem;
MIM	601883

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