Transfected Stable Cell Lines
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Cat. No. : LV05814L
Storage : -80℃ Shipping : Frozen on dry ice
Titer: Size:
| Cat. No. | LV05814L |
| Description | Lentiviral particles of ATP synthase, H+ transporting, mitochondrial Fo complex, subunit F6 (ATP5J), nuclear gene encoding mitochondrial protein, transcript variant 5. |
| Product Type | Premade Lentivirus Particles |
| Gene | ATP5J |
| Species | Human |
| Insert Size | 351 bp |
| Titer | Varies lot by lot, for example, ≥1*10^7 TU/mL, ≥1*10^8 TU/mL, ≥1*10^9 TU/mL etc. |
| Size | Varies lot by lot, for example, 100 ul, 500 ul, 1 mL etc. |
| Storage | Store at -80℃. Avoid multiple freeze/thaw cycles. |
| Shipping | Frozen on dry ice |
| Summary | Creative Biogene ensures high-quality lentivirus particles by optimizing and standardizing production protocols and performing stringent quality control (QC). The specific QC experiments performed vary between lentivirus particle lots. |
| Mycoplasma | Creative Biogene routinely tests for mycoplasma contamination using a mycoplasma detection kit. Cell lines are maintained for approximately 20 passages before being discarded and replaced with a new vial of early passage cells. Approximately 2 weeks after thawing, cell culture supernatants are tested for mycoplasma contamination. Creative Biogene ensures that lentiviral products are free of mycoplasma contamination. |
| Purity | Creative Biogene evaluates the level of impurities, such as residual host cell DNA or proteins, in prepared lentiviral vectors to ensure they meet quality standards. |
| Sterility | The lentiviral samples were inoculated into cell culture medium for about 5 days and the growth of bacteria and fungi was tested. Creative Biogene ensures that the lentiviral products are free of microbial contamination. |
| Transducibility | Upon requirement, Creative Biogene can perform in vitro or in vivo transduction assays to evaluate the ability of lentivirus to deliver genetic material into target cells, and assess gene expression and functional activities. |
| Proviral Identity Confirmation | All Creative Biogene lentiviral vectors are confirmed to have correctly integrated provirus using PCR. This test involves transducing cells with serial dilutions of the lentiviral vector, harvesting the cells a few days later, and isolating genomic DNA. This DNA is then used as a template to amplify a portion of the expected lentiviral insert. |
| Gene Name | ATP5J ATP synthase, H+ transporting, mitochondrial Fo complex, subunit F6 [ Homo sapiens ] |
| Gene Symbol | ATP5J |
| Synonyms | F6; CF6; ATP5; ATPM; ATP5A |
| Gene Description | ATP synthase, H+ transporting, mitochondrial F0 complex, subunit F6 |
| Gene ID | 522 |
| Uni Prot ID | P18859 |
| m RNA Refseq | NM_001003696.1 |
| Protein Refseq | NP_001003696.1 |
| Chromosome Location | 21q21.1 |
| Function | contributes_to ATPase activity; hydrogen ion transmembrane transporter activity; transmembrane transporter activity; transporter activity; |
| Pathway | Alzheimers disease, organism-specific biosystem; Alzheimers disease, conserved biosystem; Electron Transport Chain, organism-specific biosystem; F-type ATPase, eukaryotes, organism-specific biosystem; FOXA1 transcription factor network, organism-specific biosystem; Formation of ATP by chemiosmotic coupling, organism-specific biosystem; Huntingtons disease, organism-specific biosystem; |
| MIM | 603152 |
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