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human ATP5A1 (NM_001001937) lentivirus particles

For research use only. Not intended for any clinical use.

Cat. No. :   LV05798L

Storage :   -80℃ Shipping :   Frozen on dry ice

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Virus Particles Information

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Gene Information

Cat. No. LV05798L
Description Human ATP5A1 (NM_001001937) is cloned into the lentiviral expression vector for maximal stable delivery. The ready-to-use lentiviruses accelerate experiments investigating exogenous gene overexpression phenotypes and downstream molecular impacts.
Product Type Premade Lentivirus Particles
Gene ATP5A1
Species Human
Insert Size 1662 bp
Titer Varies lot by lot, for example, ≥1*10^7 TU/mL, ≥1*10^8 TU/mL, ≥1*10^9 TU/mL etc.
Size Varies lot by lot, for example, 100 ul, 500 ul, 1 mL etc.
Storage Store at -80℃. Avoid multiple freeze/thaw cycles.
Shipping Frozen on dry ice
Summary Creative Biogene ensures high-quality lentivirus particles by optimizing and standardizing production protocols and performing stringent quality control (QC). The specific QC experiments performed vary between lentivirus particle lots.
Mycoplasma Creative Biogene routinely tests for mycoplasma contamination using a mycoplasma detection kit. Cell lines are maintained for approximately 20 passages before being discarded and replaced with a new vial of early passage cells. Approximately 2 weeks after thawing, cell culture supernatants are tested for mycoplasma contamination. Creative Biogene ensures that lentiviral products are free of mycoplasma contamination.
Purity Creative Biogene evaluates the level of impurities, such as residual host cell DNA or proteins, in prepared lentiviral vectors to ensure they meet quality standards.
Sterility The lentiviral samples were inoculated into cell culture medium for about 5 days and the growth of bacteria and fungi was tested. Creative Biogene ensures that the lentiviral products are free of microbial contamination.
Transducibility Upon requirement, Creative Biogene can perform in vitro or in vivo transduction assays to evaluate the ability of lentivirus to deliver genetic material into target cells, and assess gene expression and functional activities.
Proviral Identity Confirmation All Creative Biogene lentiviral vectors are confirmed to have correctly integrated provirus using PCR. This test involves transducing cells with serial dilutions of the lentiviral vector, harvesting the cells a few days later, and isolating genomic DNA. This DNA is then used as a template to amplify a portion of the expected lentiviral insert.
Gene Name ATP5A1 ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1, cardiac muscle [ Homo sapiens ]
Gene Symbol ATP5A1
Synonyms OMR; ORM; ATPM; MOM2; ATP5A; hATP1; ATP5AL2
Gene Description ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1, cardiac muscle
Gene ID 498
Uni Prot ID P25705
m RNA Refseq NM_001001937.1
Protein Refseq NP_001001937.1
Chromosome Location 18q21
Function ADP binding; ATP binding; contributes_to ATPase activity; MHC class I protein binding; eukaryotic cell surface binding; hydrogen ion transporting ATP synthase activity, rotational mechanism; protein binding; proton-transporting ATPase activity, rotational mechanism; transmembrane transporter activity;
Pathway Alzheimers disease, organism-specific biosystem; Alzheimers disease, conserved biosystem; Electron Transport Chain, organism-specific biosystem; F-type ATPase, eukaryotes, organism-specific biosystem; Formation of ATP by chemiosmotic coupling, organism-specific biosystem; Huntingtons disease, organism-specific biosystem; Huntingtons disease, conserved biosystem;
MIM 164360
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