HBV-D/ayw AAV (Serotype 8)

Name: HBV-D/ayw AAV (Serotype 8)
SKU: AAVH001Z
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : AAVH001Z

Serotype : AAV Serotype 8 Storage : -80 ℃

Titer: Size:

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Virus Particles Information

Quality Control

Cat. No.	AAVH001Z
Description	AAV serotype 8 (AAV8) viral particles containing genome DNA of Hepatitis B virus (HBV) genotype D, serotype ayw.
Product Type	AAV viral particles
Serotype	AAV Serotype 8
Insert	Genome DNA of Hepatitis B virus (HBV) genotype D, serotype ayw
Applications	Cell-based HBV assays, developing HBV infection mouse models
Titer	Varies lot by lot, typically ≥1x10^12 GC/mL
Size	Varies lot by lot, for example, 30 μL, 100 μL, 500 μL etc.
Storage	Store at -80℃. Avoid multiple freeze/thaw cycles.
Shipping	Frozen on dry ice

Summary	Creative Biogene ensures high-quality AAV particles by optimizing and standardizing production protocols and performing stringent quality control (QC). The specific QC experiments performed vary between AAV particle lots.
Endotoxin	Endotoxins, primarily derived from Gram-negative bacteria, can trigger adverse immune responses. Endotoxin contamination is a significant concern in the production of AAV, especially for applications in animal studies and gene therapy. Effective endotoxin quality control is essential in the development and manufacturing of AAV particles. Creative Biogene utilizes rigorous endotoxin detection methods to monitor the endotoxin level in our produced AAV particles to ensure regulatory compliance.
Purity	AAV purity is critical for ensuring the safety and efficacy of AAV-based applications.AAV capsids are composed of three main protein components, known as viral proteins: VP1, VP2, and VP3. These proteins play a critical role in the structure and functionality of the AAV capsid. Monitoring the VP1, VP2, and VP3 content in AAV preparations is essential for quality control in AAV production. Our AAV particles are tested for showing three clear bands of VP1, VP2 VP3 by SDS-PAGE.
Sterility	The AAV virus samples are inoculated into the cell culture medium for about 5 days to detect bacterial and fungal growth.
Transducibility	Upon requirement, Creative Biogene can perform in vitro or in vivo transduction assays to evaluate the ability of AAV to deliver genetic material into target cells or tissues, and assess gene expression and functional activities.
Empty vs. Full Capsids	Based-on our proprietary AAV production and purification technology, Creative Biogene can always offer AAV particles with high ratio of full capsids. If required, we can also assess the ratio for a specifc lot of AAV particles by transmission electron microscopy (TEM) or other methods.

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Background

Applications

Q & A

Customer Reviews

Hepatitis B virus (HBV) is a member of the Hepadnaviridae family of viruses. The genome of HBV is made of a partially double-stranded DNA virus. There are four known genes encoded by the genome called C, P, S, and X. HBV is divided into four major serotypes (adr, adw, ayr, ayw) based on antigenic epitopes present on its envelope proteins, and is divided into ten genotypes (A to J) and forty subgenotypes according to overall nucleotide sequence variation of the genome. HBV infection can cause the disease hepatitis B. Hepatitis B can be acute and later become chronic, leading to other diseases and health conditions. Infection with HBV can also lead to cirrhosis and hepatocellular carcinoma, and increase the risk of pancreatic cancer.

AAV serotype 8 (AAV8) viral particles contain genomic DNA of Hepatitis B virus (HBV) genotype D, serotype ayw. These types of vectors are often used in gene therapy research and applications because of their ability to deliver genetic material into host cells. Here are some potential applications of this type of construct:

Gene therapy for liver disease: Since AAV serotype 8 has a high tropism for hepatocytes, this construct can be used to deliver therapeutic genes to the liver, potentially treating inherited liver diseases such as hemophilia B, alpha-1-antitrypsin deficiency, etc.

Chronic hepatitis B treatment: If the HBV element refers to a sequence from the hepatitis B virus, it may be designed to interfere with viral replication or gene expression. This hybrid vector may be used to deliver antiviral genes or RNA interference molecules that target HBV RNA.

Vaccine development: The HBV component may elicit an immune response. This hybrid AAV may be used as a vaccine vector to induce immunity by including multiple antigens, not only against HBV, but also against other pathogens.

Cancer therapy: Certain cancers, especially hepatocellular carcinoma (HCC), are closely associated with chronic HBV infection. This construct can be designed to deliver therapeutic genes to combat HBV-associated HCC.

Customer Q&As

How are virus titers determined?

A: There are 3 commonly used protocols for determining adenovirus titer: (1) OD260 Assay, (2) Plaque Formation Assay, and (3) End-point Dilution Assay.

Does rAAV have long-term transduction?

A: Yes.Over the past 20 years, rAAV has been commonly used as a tool for efficient and long-term gene expression in basic research and clinical gene therapy. For example, rAAV induced transgene expression in non-human primate muscle tissue can last for more than 10 years.

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Customer Reviews

A Good Product for Preparing Mouse Models of HBV Infection

Creative Biogene's AAV-HBV carries 1.3× the full-length genome of HBV, and a mouse model of persistent HBV infection can be prepared by a single tail vein injection of AAV-HBV. It has the advantages of simple preparation, high success rate, homogeneity, stability, characterization of good dose-effect relationship and wide range of applications.

United States

2022-03-19

Proven Good Product

The HBV-AAV mouse model has been validated for use in a wide range of HBV drug evaluations and vaccine screening.

United States

2022-07-18

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HBV-D/ayw AAV (Serotype 8)

Virus Particles Information

Quality Control

Background

Applications

Q & A

Customer Reviews

How are virus titers determined?

Does rAAV have long-term transduction?

A Good Product for Preparing Mouse Models of HBV Infection

Proven Good Product

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