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hSLC5A5/Luc Reporter Cell Line-A549

hSLC5A5/Luc Reporter Cell Line-A549

Cat.No. :  CSC-RR0260 Host Cell:  A549

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Cell Line Information

Cell Culture Information

Safety and Packaging

Cat. No. CSC-RR0260
Description hSLC5A5/Luc Reporter Cell Line-A549 is engineered to stably co-express human SLC5A5 and luciferase reporter gene. It is an ideal positive control for use in iodine uptake and bioluminescence assays. In addition, A549 cells can form tumors in mouse lungs. The in vivo growth of these tumors can be monitored using PET/SPECT imaging or bioluminescent imaging.
Gene hSLC5A5/Luciferase
Host Cell A549
Host Cell Species Homo sapiens (Human)
Stability Validated for at least 10 passages
Application

1. Gene expression studies

2. Protein localization

3. Drug screening and toxicology

4. Live cell imaging

Quality Control Negative for bacteria, yeast, fungi and mycoplasma.
Shipping Dry ice
Storage Liquid nitrogen
Revival Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Mycoplasma Negative
Format One frozen vial containing millions of cells
Storage Liquid nitrogen
Safety Considerations

The following safety precautions should be observed.

1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.

2. No eating, drinking or smoking while handling the stable line.

3. Wash hands after handling the stable line and before leaving the lab.

4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells.

5. All waste should be considered hazardous.

6. Dispose of all liquid waste after each experiment and treat with bleach.

Ship Dry ice
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