GFP-Actin Reporter Cell Line - HT22

Name: GFP-Actin Reporter Cell Line - HT22
SKU: CSC-RR01266
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RR01266

Host Cell : HT-22 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Cat. No.	CSC-RR01266
Description	This cell line is engineered to stably express GFP-Actin fusion gene in HT22 cells.
Target Gene	GFP-Actin
Host Cell	HT-22
Host Cell Species	Mus musculus (Mouse)
Applications	This cell line stably expresses fluorescent GFP fused to actin, enabling real-time, live-cell visualization of actin cytoskeleton dynamics without cell fixation or permeabilization. Cytoskeleton morphology research Track actin filament assembly/disassembly, stress fiber formation, lamellipodia, filopodia and podosome structure under normal or stimulated conditions. Cell migration & invasion assays Quantify dynamic actin remodeling at leading edges; evaluate how drugs, cytokines or gene manipulations alter cell motility and metastatic capacity. Cell adhesion study Monitor actin rearrangement during focal adhesion assembly and cell spreading on different extracellular matrix substrates. Drug screening for cytoskeleton-targeting compounds High-throughput live-cell imaging to screen small molecules that modulate actin polymerization, cytoskeletal stability or cell movement. Mechanobiology analysis Detect actin cytoskeleton responses to mechanical stimuli like shear flow, substrate stiffness or stretching force.
Size	One vial of frozen cells, typically >1x10^6cells/vial
Stability	This cell line is stable at least 10 passages.
Quality Control	Imaging of cells under fluorescent microscopy Mycoplasma detection.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties	Adherent cell line

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

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