Firefly Luciferase mRNA (AG (3' OMe) capped, Cy5-UTP & 5-Methoxy-UTP modified)

Name: Firefly Luciferase mRNA (AG (3' OMe) capped, Cy5-UTP & 5-Methoxy-UTP modified)
SKU: PMRN-0304
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : PMRN-0304

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Product Information

Cat. No.	PMRN-0304
Description	The Firefly Luciferase mRNA can express luciferase protein, which has been initially extracted from the Photinus pyralis (Firefly), once it has been transfected into cells. Firefly Luciferase is a commonly used bioluminescent reporter for gene regulation and function study. It is applicable in various assays such as gene expression, cell viability, and in vivo imaging. AG (3' OMe) cap is used for co-transcriptional capping of mRNA to form Cap 1 structure, m7(3'OMeG)(5')ppp(5')(2'OMeA)pG. This cap obtains the ability only to insert in the proper orientation which ensures AG (3' OMe)-capped mRNA with high translation efficacy. Meanwhile, the incorporation of 5-Methoxy-UTP and Cy5-UTP made the resulting mRNA with less immunogenicity and easy to visualize.
Features	• mRNA synthesized on error free sequence verified plasmid DNA template • Cap1 capping and poly-A tail incorporated • mRNA modified with 5-Methoxy-UTP and Cy5-UTP • Degrades the DNA template after RNA synthesis with DNase
Sequence	mRNA Length: 1921 base
Storage	Store at or below -70°C. Avoid repeated freeze/thaw cycles. Aliquot if necessary using RNase-free equipment, reagents, pipet tips, tubes, and containers.

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