Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : PMRN-0301
| Cat. No. | PMRN-0301 |
| Description | The EGFP mRNA can express an enhanced green fluorescence once it has been transfected into cells. This is commonly used as a control to study transfection and expression in mammalian cells using fluorescence-based assays. AG (3' OMe) cap is used for co-transcriptional capping of mRNA to form Cap 1 structure, m7(3'OMeG)(5')ppp(5')(2'OMeA)pG. This cap obtains the ability only to insert in the proper orientation which ensures AG (3' OMe)-capped mRNA with high translation efficacy. Meanwhile, the incorporation of 5-Methoxy-UTP and Cy5-UTP made the resulting mRNA with less immunogenicity and easy to visualize. |
| Features |
• mRNA synthesized on error free sequence verified plasmid DNA template • Cap1 capping and poly-A tail incorporated • mRNA modified with 5-Methoxy-UTP and Cy5-UTP • Degrades the DNA template after RNA synthesis with DNase |
| Sequence | mRNA Length: 996 base |
| Storage | Store at or below -70°C. Avoid repeated freeze/thaw cycles. Aliquot if necessary using RNase-free equipment, reagents, pipet tips, tubes, and containers. |
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