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EGFP mRNA (AG (3' OMe) capped, Cy5-UTP & 5-Methoxy-UTP modified)

For research use only. Not intended for any clinical use.

Cat. No. :   PMRN-0301

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Cat. No. PMRN-0301
Description The EGFP mRNA can express an enhanced green fluorescence once it has been transfected into cells. This is commonly used as a control to study transfection and expression in mammalian cells using fluorescence-based assays. AG (3' OMe) cap is used for co-transcriptional capping of mRNA to form Cap 1 structure, m7(3'OMeG)(5')ppp(5')(2'OMeA)pG. This cap obtains the ability only to insert in the proper orientation which ensures AG (3' OMe)-capped mRNA with high translation efficacy. Meanwhile, the incorporation of 5-Methoxy-UTP and Cy5-UTP made the resulting mRNA with less immunogenicity and easy to visualize.
Features • mRNA synthesized on error free sequence verified plasmid DNA template
• Cap1 capping and poly-A tail incorporated
• mRNA modified with 5-Methoxy-UTP and Cy5-UTP
• Degrades the DNA template after RNA synthesis with DNase
Sequence mRNA Length: 996 base
Storage Store at or below -70°C. Avoid repeated freeze/thaw cycles. Aliquot if necessary using RNase-free equipment, reagents, pipet tips, tubes, and containers.
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