Dicots SaCas9 Genome Editing Kit (Hygromycin)

Name: Dicots SaCas9 Genome Editing Kit (Hygromycin)
SKU: CC-18
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CC-18

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Product Information

Cat. No.	CC-18
Description	All-in-one, ready-to-use SaCas9 and guide RNA (gRNA) expression plasmids for use with dicots, especially with Arabidopsis. The Dicots SaCas9 Genome Editing Kit is a highly effective and accurate gene editing kit which is based on the latest artificial endonuclease CRISPR/SaCas9.
Efficiency	SaCas9 expression cassette and sgRNA expression cassette are combined into one vector which offers a higher transformation efficiency.
Features	Two functional components are combined into one vector which offers a higher transfection efficiency. High expression level of SaCas9 protein in dicots by using CAMV 35S promoter and dicot codon optimized-SaCas9. High activity of sgRNA in Arabidopsis by using AtU6 (Arabidopsis U6) promoter. Mutiplex genome editing
Applications	The Dicots SaCas9 Genome Editing Kit is applicable to modify genes in almost all kinds of dicots.
Resistance	Kanamycin
Selection Marker	Hygromycin B

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Customer Q&As

What is Hygromycin?

A: Hygromycin phosphotransferase (Hpt) is the most widely used selection marker which confers tolerance to the herbicide hygromycin because several crops have a natural tolerance to kanamycin.

What are the categories of CRISPR/CAS system?

A: The CRISPR/Cas systems are divided into two classes. The Class 1 systems have multiple Cas protein subunits, while the Class 2 systems utilize a single, multifunctional protein effector. Class 2 CRISPR systems are further divided into types II, V, and VI. In addition to the commonly used type II effector SpCas9, other orthologs with RNA-guided site-specific nuclease (SSN) activity have been engineered for use as tools in the genome editing of eukaryotic cells. These orthologs include proteins from Staphylococcus aureus (SaCas9), Francisella novicida (FnCas9), Neisseria meningitidis (NmCas9), Streptococcus thermophilus (St1Cas9), Brevibacillus laterosporus (BlCas9), and Campylobacter jejuni (CjCas9).

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Customer Reviews

Worked Very Well

SaCas9 is very suitable for soybean genome editing. We employed the Monocots SaCas9 Genome Editing Kit to successfully induce targeted mutations in five target sites of two soybean genes, GmFT2a and GmFT5a.

United States

2021-12-02

I recommend it

I recommend it. Dicots SaCas9 Genome Editing Kit has helped me to achieve site-specific knockout of target genes in hairy roots and regenerated plants of soybean to improve important agronomic characteristics.

United States

2022-02-06

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Dicots SaCas9 Genome Editing Kit (Hygromycin)

Product Information

Q & A

Customer Reviews

What is Hygromycin?

What are the categories of CRISPR/CAS system?

Worked Very Well

I recommend it

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