Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : CC-20
| Cat. No. | CC-20 |
| Description | All-in-one, ready-to-use SaCas9 and guide RNA (gRNA) expression plasmids for use with dicots, especially with Arabidopsis. The Dicots SaCas9 Genome Editing Kit is a highly effective and accurate gene editing kit which is based on the latest artificial endonuclease CRISPR/SaCas9. |
| Efficiency | SaCas9 expression cassette and sgRNA expression cassette are combined into one vector which offers a higher transformation efficiency. |
| Features |
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| Applications | The Dicots SaCas9 Genome Editing Kit is applicable to modify genes in almost all kinds of dicots. |
| Resistance | Kanamycin |
| Selection Marker | GFP |
A: SaCas9 and its engineered variant SaKKH (E782K/N968K/R1015H), which relaxes the canonical NNGRRT PAM of SaCas9 to NNNRRT, have been used to achieve the efficient targeted mutagenesis of Arabidopsis thaliana, rice, tobacco (Nicotiana tabacum), and citrus (Citrus sp.).
A: SaCas9 recognizes a 5′-NNGRRT-3′ (where R represents A or G) PAM sequence, which is distinct from that of SpCas9 and provides additional potential target sequences.
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SaCas9 can direct highly specific genome editing activity, and it will therefore be useful for expanding the scope of potential target genes for genome editing in soybean.
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