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A: SaCas9 and its engineered variant SaKKH (E782K/N968K/R1015H), which relaxes the canonical NNGRRT PAM of SaCas9 to NNNRRT, have been used to achieve the efficient targeted mutagenesis of Arabidopsis thaliana, rice, tobacco (Nicotiana tabacum), and citrus (Citrus sp.).
A: SaCas9 recognizes a 5′-NNGRRT-3′ (where R represents A or G) PAM sequence, which is distinct from that of SpCas9 and provides additional potential target sequences.
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
SaCas9 can direct highly specific genome editing activity, and it will therefore be useful for expanding the scope of potential target genes for genome editing in soybean.
United States
01/18/2023
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