Dicots Cpf1 Genome Editing Kit (Hygromycin)

Name: Dicots Cpf1 Genome Editing Kit (Hygromycin)
SKU: CC-26
Rating: 4.0 (1 reviews)

For research use only. Not intended for any clinical use.

Cat.No.

CC-26

Description

All-in-one, ready-to-use Cpf1 and crRNA expression plasmids for use with monocots, especially with rice. The Dicots Cpf1 Genome Editing Kit is a highly effective and accurate gene editing kit which is based on the latest artificial endonuclease CRISPR/Cpf1.

Applications

The Dicots Cpf1 Genome Editing Kit is applicable to modify genes in almost all kinds of dicots.

Efficiency

Cpf1 expression cassette and sgRNA expression cassette are combined into one vector which offers a higher transformation efficiency.

Features

•Two functional components are combined into one vector which offers a higher transfection efficiency.
•High expression level of Cpf1 protein in Monocots by using Ubiquitin promoter and Dicot codon optimized-Cpf1.
•High activity of sgRNA in Rice by using AtU6 (Arabidopsis U6) promoter.
•Mutiplex genome editing

Resistance

Kanamycin

Selection Marker

Hygromycin B

Publications

Q & A

Customer Reviews

Customer Q&As

What are the characteristics of CRISPR-Cpf1 composition?

A: The composition of CRISPR-Cpf1 is different from the most commonly used CRISPR-SpCas9. The difference is that the Cpf1 system includes Cpf1 protein and CRISPR RNA (crRNA), but does not contain trans-activating CRISPR RNA (tracrRNA) sequence, thus eliminating the step of tracrRNA catalyzing the maturation of CRISPR RNA precursor (pre-crRNA).

What is the site of CRISPR-Cpf1 cutting target DNA?

A: Unlike Cas9, which uses the RuvC and HNH domains to cut the blunt ends in the seed region, Cpf1 uses the RuvC and Nuc domains to cut the 23 complementary strands and the 18 non-complementary strands of the target DNA outside the seed region. The product of cross-cutting is the sticky ends. In addition to cutting the 23 positions of the target DNA complementary chain, Cpf1 can form multiple cutting sites from 14 to 18 positions of the non-complementary chain.

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Customer Reviews

Worked Very Well

Great kit. Using Dicots Cpf1 Genome Editing Kit, I have successfully edited two genes that changed the fat content in soybean oil.

United States

12/02/2021

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Dicots Cpf1 Genome Editing Kit (Hygromycin)

Publications

Q & A

Customer Reviews

What are the characteristics of CRISPR-Cpf1 composition?

What is the site of CRISPR-Cpf1 cutting target DNA?

Worked Very Well

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