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Arabidopsis CRISPR/Cas9 Genome Editing Kit (Kanamycin)

For research use only. Not intended for any clinical use.
Cat.No.
CC-17
Description
The Arabidopsis CRISPR/Cas9 Genome Editing Kit is a highly effective and universal gene editing kit. It is intended for Agrobacterium-mediated plant transformation. The CRISPR construct contains a sgRNA expression cassette driven by AtU6 promoter, and a codon optimized Cas9 expression cassette driven by CAMV 35S promoter. The Arabidopsis CRISPR/Cas11 Genome Editing Kit is applicable to modify genes in almost all kinds of dicots, especially in Arabidopsis.
Applications
The Arabidopsis CRISPR/Cas9 Genome Editing Kit is applicable to modify genes in almost all kinds of dicots, especially in Arabidopsis.
Efficiency
Cas9 expression cassette and sgRNA expression cassette are combined into one vector which offers a higher transformation efficiency.
Features
•Two functional components are combined into one vector which offers a higher transfection efficiency.
•High expression level of Cas9 protein in monocots by using maize ubiquitin promoter and Dicot codon optimized-Cas9.
•High activity of sgRNA in rice by using AtU6 (Arabidopsis U6) promoter.
•Mutiplex genome editing
Resistance
Kanamycin
Selection Marker
Kanamycin

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Q & A

Customer Reviews

Customer Q&As
What is Kanamycin?

A: Kanamycin belongs to the class of medicines known as aminoglycoside antibiotics. It works by killing bacteria or preventing their growth and is a kind of marker gene widely used in transgenic plants.

What are the advantages of CRISPR/Cas9 in multiplex editing?

A: Multiplex editing of the CRISPR/Cas9 system simply requires the monomeric Cas9 protein and any number of different sequence-specific gRNAs. In contrast, multiplex editing with ZFNs or TALENs requires separate dimeric proteins specific for each target site.

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Customer Reviews
Reliable

The two functional components are combined into a carrier to provide higher transfection efficiency. It significantly shortened my experimental time.

United States

01/18/2023

Fast & Convenient

The delivery speed is fast. I soon received the Arabidopsis CRISPR/Cas9 Genome Editing Kit product.

United States

02/21/2023

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