Arabidopsis CRISPR/Cas9 Genome Editing Kit (Basta)

Name: Arabidopsis CRISPR/Cas9 Genome Editing Kit (Basta)
SKU: CC-16
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CC-16

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Cat. No.	CC-16
Description	The Arabidopsis CRISPR/Cas9 Genome Editing Kit is a highly effective and universal gene editing kit. It is intended for Agrobacterium-mediated plant transformation. The CRISPR construct contains a sgRNA expression cassette driven by AtU6 promoter, and a codon optimized Cas9 expression cassette driven by CAMV 35S promoter. The Arabidopsis CRISPR/Cas10 Genome Editing Kit is applicable to modify genes in almost all kinds of dicots, especially in Arabidopsis.
Efficiency	Cas9 expression cassette and sgRNA expression cassette are combined into one vector which offers a higher transformation efficiency.
Features	Two functional components are combined into one vector which offers a higher transfection efficiency. High expression level of Cas9 protein in monocots by using maize ubiquitin promoter and Dicot codon optimized-Cas9. High activity of sgRNA in rice by using AtU6 (Arabidopsis U6) promoter. Mutiplex genome editing
Applications	The Arabidopsis CRISPR/Cas9 Genome Editing Kit is applicable to modify genes in almost all kinds of dicots, especially in Arabidopsis.
Resistance	Kanamycin
Selection Marker	Basta

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What are the advantages of Arabidopsis as a model organism?

A: Arabidopsis is a self-pollinating plant. Its genome is very small in higher plants. The gene is highly homozygous and the mutation rate is high when treated with physical and chemical factors. It is easy to obtain various metabolic function defects. It is a good material for genetic research and is known as "Drosophila in plants" by scientists.

What are the disadvantages of Arabidopsis as a model organism?

A: There are mainly two aspects as follows: (1) Arabidopsis is an annual herb in the Cruciferae family, which differs greatly from other species in physiology; (2) Arabidopsis genome is small, and other plant genomes are large, so the molecular model obtained from Arabidopsis is usually not applicable to other plants.

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Reliable

A codon optimized Cas9 protein and a gRNA are expressed from a single vector and provided as ready-to-use, transfection-grade DNA.

United States

2022-02-06

Useful Tool

Using this Arabidopsis CRISPR/Cas9 Genome Editing Kit, I successfully achieved mutiplex genome editing in Arabidopsis. I like it.

United States

2022-09-10

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Arabidopsis CRISPR/Cas9 Genome Editing Kit (Basta)

Product Information

Q & A

Customer Reviews

What are the advantages of Arabidopsis as a model organism?

What are the disadvantages of Arabidopsis as a model organism?

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