Transfected Stable Cell Lines
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Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : CLOE-1903 Host Cell : HEK293
| Cat. No. | CLOE-1903 |
| Description | This cell lysate overexpresses rat Col4a3 (Gly1426-His1670) with the Fc region of human IgG1 at the N-terminus. Purification of target protein from this cell lysate has been verified. |
| Introduction | This cell lysate overexpresses rat Col4a3 (Gly1426-His1670) with the Fc region of human IgG1 at the N-terminus. Purification of target protein from this cell lysate has been verified. |
| Target Gene | Col4a3 |
| Host Cell | HEK293 |
| Host Cell Species | Homo sapiens (Human) |
| Applications | Positive control for Western Blot (WB), antibody verification, protein purification. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Storage | Upon receipt, store at 4℃ , or prepare aliquots and store at -80℃. Multiple freeze-thaw cycles should be avoided. |
| Shipping | Ice package |
| Gene Name | Col4a3 collagen, type IV, alpha 3 [ Mus musculus ] |
| Gene Symbol | Col4a3 |
| Synonyms | [a]3(IV); alpha3(IV) |
| Gene Description | procollagen, type IV, alpha 3 |
| GeneID | 12828 |
| Uni ProtID | Q9QZS0 |
| mRNA Refseq | NM_007734.2 |
| Protein Refseq | NP_031760.2 |
| Chromosome Location | 1 C5; 1 |
| Function | extracellular matrix structural constituent; integrin binding; |
| Pathway | mRNA processing, organism-specific biosystem; |
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