RFP Stable Cell Line-HepG2

Name: RFP Stable Cell Line-HepG2
SKU: CSC-RR0126

Cat.No. : CSC-RR0126 Host Cell: HepG2

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Cell Line Information

Cell Culture Information

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Cat. No.	CSC-RR0126
Description	Hep G2 is a perpetual cell line which was derived from the liver tissue of a 15-year-old Caucasian American male with a well-differentiated hepatocellular carcinoma. These cells are epithelial in morphology, have a modal chromosome number of 55, and are not tumorigenic in nude mice. Hep G2 cells express 3-hydroxy-3-methylglutaryl-CoA reductase and hepatic triglyceride lipase activities. Hep G2 cells demonstrate decreased expression of apoA-I mRNA and increased expression of catalase mRNA in response to gramoxone (oxidative stress). There is no evidence of a Hepatitis B virus genome in this cell line. The RFP Stable Cell Line-HepG2 constitutively expresses RFP.
Host Cell	HepG2
Host Cell Species	Homo sapiens (Human)
Stability	Validated for at least 10 passages
Reporter Type	Fluorescent protein
Application	1. Gene expression studies 2. Protein localization 3. Drug screening and toxicology 4. Live cell imaging
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Recommended Medium	Inquiry for instruction of culturing

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm² flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

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