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Cat.No. : CSC-RT0110
Host Cell: MC3T3-E1 Target Gene: Prdx1
Size: 1x10^6 cells/vial, 1mL Validation: Sequencing
| Cat. No. | CSC-RT0110 |
| Description | MC3T3-E1-Prdx1 cell line is a stable cell line with a homozygous knockout of Prdx1 |
| Target Gene | Prdx1 |
| Host Cell | MC3T3-E1 |
| Host Cell Species | Mus musculus (Mouse) |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.
A: Single clonal cell.
A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.
A: The cell line should be stored in liquid nitrogen for long-term preservation.
A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
I am incredibly impressed with the performance of Prdx1 Knockout Cell Line-MC3T3-E1. Its exceptional knockout efficiency and consistent results have been instrumental in advancing my research and understanding the functional role of specific genes.
France
04/11/2023
Using Prdx1 Knockout Cell Line-MC3T3-E1 has been a outstanding competent assistant for my studies. Its user-friendly workflow and reliable knockout outcomes have streamlined the gene editing process, allowing me to focus more on data analysis and interpretation.
United States
05/09/2023
Prdx1 Knockout Cell Line-MC3T3-E1 has exceeded my expectations in terms of knockout efficiency and ease of use. Its robust performance has enabled me to generate reliable knockout models, providing valuable insights into gene function.
United States
08/23/2020
I can't stress enough the fact that the value of Prdx1 Knockout Cell Line-MC3T3-E1 in my research endeavors. Its high success rate and reproducible knockout outcomes have been critical in unraveling the complex interplay between genes and their biological functions.
United States
05/12/2021
I wholeheartedly recommend Prdx1 Knockout Cell Line-MC3T3-E1 to fellow researchers. Its exceptional performance, reliability, and user-friendly design have significantly contributed to the success and impact of my experiments.
United States
08/15/2021
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