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Prdx1 Knockout Cell Line-MC3T3-E1

For research use only. Not intended for any clinical use.

Cat. No. :   CSC-RT0110

Target Gene :   Prdx1 Host Cell :   MC3T3-E1

Size :   >1x106 cells/vial Validation :   Sequencing

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No. CSC-RT0110
Description MC3T3-E1-Prdx1 cell line is a stable cell line with a homozygous knockout of Prdx1
Target Gene Prdx1
Host Cell MC3T3-E1
Host Cell Species Mus musculus (Mouse)
Size >1x106 cells/vial
Validation Sequencing
Revival Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Mycoplasma Negative
Format One frozen vial containing millions of cells
Storage Liquid nitrogen
Safety Considerations The following safety precautions should be observed.
1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.
2. No eating, drinking or smoking while handling the stable line.
3. Wash hands after handling the stable line and before leaving the lab.
4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells.
5. All waste should be considered hazardous.
6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship Dry ice
Target Gene PRDX1
Background This gene encodes a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. The encoded protein may play an antioxidant protective role in cells, and may contribute to the antiviral activity of CD8(+) T-cells. This protein may have a proliferative effect and play a role in cancer development or progression. Four transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq, Jan 2011]
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Q & A

Customer Reviews

Customer Q&As
How is the knockout cell line validated?

A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.

Is the product a single clonal cell or mixed cell pool?

A: Single clonal cell.

Can I confirm gene knockout by RT-qPCR?

A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.

How can I store the cell product?

A: The cell line should be stored in liquid nitrogen for long-term preservation.

Is it possible to get multiple knockout clones for my GOI?

A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.

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Customer Reviews
Consistent results

I am incredibly impressed with the performance of Prdx1 Knockout Cell Line-MC3T3-E1. Its exceptional knockout efficiency and consistent results have been instrumental in advancing my research and understanding the functional role of specific genes.

France

User-friendly workflow

Using Prdx1 Knockout Cell Line-MC3T3-E1 has been a outstanding competent assistant for my studies. Its user-friendly workflow and reliable knockout outcomes have streamlined the gene editing process, allowing me to focus more on data analysis and interpretation.

United States

Generate reliable knockout models

Prdx1 Knockout Cell Line-MC3T3-E1 has exceeded my expectations in terms of knockout efficiency and ease of use. Its robust performance has enabled me to generate reliable knockout models, providing valuable insights into gene function.

United States

Reproducible knockout outcomes

I can't stress enough the fact that the value of Prdx1 Knockout Cell Line-MC3T3-E1 in my research endeavors. Its high success rate and reproducible knockout outcomes have been critical in unraveling the complex interplay between genes and their biological functions.

United States

Exceptional performance

I wholeheartedly recommend Prdx1 Knockout Cell Line-MC3T3-E1 to fellow researchers. Its exceptional performance, reliability, and user-friendly design have significantly contributed to the success and impact of my experiments.

United States

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