Panoply™ Human TNFSF13 Over-expressing Stable Cell Line

Name: Panoply™ Human TNFSF13 Over-expressing Stable Cell Line
SKU: CSC-SC016375
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-SC016375

Host Cell : HEK293 (CHO and other cell types are also available) Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

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Gene Information

Cat. No.	CSC-SC016375
Description	Using Creative Biogene's proprietary lentiviral vectors, we subclone the target gene into lentivector, generate the lentivirus particles, sequentially infect the cell line HEK293 (other cell types are also available according to your requirements), and select the clones constantly expressing target gene at high level.
Target Gene	TNFSF13
Gene Species	Homo sapiens (Human)
Host Cell	HEK293 (CHO and other cell types are also available)
Host Cell Species	Species varies
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research
Size	2 × 10^6 cells / vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry Ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	TNFSF13 tumor necrosis factor (ligand) superfamily, member 13 [ Homo sapiens ]
Gene Symbol	TNFSF13
Synonyms	TNFSF13; tumor necrosis factor (ligand) superfamily, member 13; tumor necrosis factor ligand superfamily member 13; APRIL; CD256; a proliferation-inducing ligand; tumor necrosis factor-like protein ZTNF2; tumor necrosis factor-related death ligand-1; TNF- and APOL-related leukocyte expressed ligand 2; TALL2; ZTNF2; TALL-2; TRDL-1; FLJ57090; UNQ383/PRO715;
GeneID	8741
Uni ProtID	O75888
mRNA Refseq	BC008042
Chromosome Location	17p13.1
Function	cytokine activity; receptor binding;
Pathway	Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Gene Expression, organism-specific biosystem; Intestinal immune network for IgA production, organism-specific biosystem; Intestinal immune network for IgA production, conserved biosystem; Regulation of mRNA Stability by Proteins that Bind AU-rich Elements, organism-specific biosystem; Rheumatoid arthritis, organism-specific biosystem;
MIM	604472

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A Proliferation-Inducing Ligand (APRIL, also known as TNFSF13 or CD256) is a member of the tumor necrosis factor (TNF) superfamily and is closely associated with the pathogenesis and progression of various diseases. In this study, researchers investigated the potential link between APRIL expression and Helicobacter pylori (H. pylori) infection in gastric cancer patients, as well as the underlying mechanisms. The study found a positive correlation between APRIL expression levels and H. pylori infection. The results demonstrated that the downregulation of miR-145 occurred in a dose- and time-dependent manner in response to H. pylori infection, mirroring the expression pattern of APRIL. In SGC7901 and BGC823 cell lines, miR-145 significantly attenuated the impact of H. pylori infection on APRIL gene expression. Further experiments revealed that APRIL overexpression promoted the proliferation, migration, invasion, and metastasis of gastric cancer cells while inhibiting apoptosis; conversely, APRIL knockdown suppressed these effects. These findings indicate that APRIL activates the classical NF-κB signaling pathway by promoting AKT phosphorylation.

To investigate the role of APRIL in cell proliferation and viability, researchers selected two human gastric cancer (GC) cell lines-an APRIL-knockdown line (SGC7901-shAPRIL) and an APRIL-overexpressing line (AGS-APRIL)-for further study. Western blot analysis confirmed the successful modulation of APRIL expression levels in these cell lines (Figure 1a, b). CCK-8 assays conducted over a 7-day period (days 1–7) revealed that APRIL knockdown significantly inhibited the growth of SGC7901 cells (Figure 1c), whereas APRIL overexpression significantly promoted the proliferation of AGS cells (Figure 1d). Colony formation assays further corroborated these findings (Figure 1e, f). Subsequently, the researchers assessed the viability of the gastric cancer cells with modulated APRIL expression using flow cytometry. As shown in Figure 1g, the proportion of apoptotic cells was significantly increased in the APRIL-knockdown gastric cancer cell line, while it was significantly decreased in the APRIL-overexpressing gastric cancer cell line.

Figure 1. APRIL contributed to proliferation and prevented apoptosis of GC cells. (Zhang Q, et al., 2021)

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