Panoply™ Human TNFSF11 Over-expressing Stable Cell Line

Name: Panoply™ Human TNFSF11 Over-expressing Stable Cell Line
SKU: CSC-SC016372
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-SC016372

Host Cell : HEK293 (CHO and other cell types are also available) Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

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Gene Information

Cat. No.	CSC-SC016372
Description	Using Creative Biogene's proprietary lentiviral vectors, we subclone the target gene into lentivector, generate the lentivirus particles, sequentially infect the cell line HEK293 (other cell types are also available according to your requirements), and select the clones constantly expressing target gene at high level.
Target Gene	TNFSF11
Gene Species	Homo sapiens (Human)
Host Cell	HEK293 (CHO and other cell types are also available)
Host Cell Species	Species varies
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research
Size	2 × 10^6 cells / vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry Ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	TNFSF11 tumor necrosis factor (ligand) superfamily, member 11 [ Homo sapiens ]
Gene Symbol	TNFSF11
Synonyms	TNFSF11; tumor necrosis factor (ligand) superfamily, member 11; tumor necrosis factor ligand superfamily member 11; CD254; ODF; OPGL; RANKL; TRANCE; osteoprotegerin ligand; osteoclast differentiation factor; TNF-related activation-induced cytokine; receptor activator of nuclear factor kappa B ligand; receptor activator of nuclear factor kappa-B ligand; sOdf; OPTB2; hRANKL2;
Gene ID	8600
Uni Prot ID	O14788
m RNA Refseq	BC117286
Chromosome Location	13q14
Function	cytokine activity; cytokine activity; receptor activity; tumor necrosis factor receptor
Pathway	Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; IL6-mediated signaling events, organism-specific biosystem; Osteoblast Signaling, organism-specific biosystem; Osteoclast Signaling, organism-specific biosystem; Osteoclast differentiation, organism-specific biosystem; Osteoclast differentiation, conserved biosystem;
MIM	602642

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Lung adenocarcinoma (LUAD) is the most common and deadliest subtype of lung cancer, and remains a major global health problem. Despite advances in targeted therapy and immunotherapy, only a small percentage of patients benefit. Therefore, new treatment strategies are urgently needed to improve the treatment outcomes of lung cancer. Here, researchers revealed the overexpression of TNFSF11 in lung adenocarcinoma patients and its negative correlation with peroxisome-associated enzymes. Through gene knockdown or overexpression experiments, a negative correlation between TNFSF11 and GPX4 was found. Furthermore, cells overexpressing TNFSF11 were more sensitive to ferroptosis inducers. These studies provide valuable insights into the role of TNFSF11, revealing its negative regulatory effect on GPX4, which may have significant implications for developing new treatment strategies.

GPX4 is a key regulator of ferroptosis, a unique form of iron-dependent non-apoptotic cell death. Inhibition of GPX4 leads to lipid peroxidation, which in turn triggers ferroptosis. Therefore, cells with elevated GPX4 levels are resistant to ferroptosis. Conversely, cancer cells with low GPX4 expression levels may be more susceptible to ferroptosis inducers. To test this hypothesis, researchers treated TNFSF11-overexpressing cells and control cells with different concentrations of erastin and RSL3 (both ferroptosis inducers). The results showed that TNFSF11-overexpressing cells were more sensitive to these inducers, which may be related to their reduced GPX4 expression levels (Figure 1A-D). Notably, the inhibitory effects of these inducers on cell growth could be antagonized by the ferroptosis inhibitor ferrostatin-1 (Figure 1E-H). These observations suggest that ferroptosis inducers may offer a promising therapeutic approach for targeting cancers that express TNFSF11. ROS detection experiments further confirmed that erastin could induce an increase in ROS levels in TNFSF11-overexpressing cells within 4 hours, while ferrostatin-1 could inhibit the production of this ROS (Figure 1I-L).

Figure 1. TNFSF11 overexpressing cells are more susceptible to ferroptosis inducers. (Li Z, et al., 2024)

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