Panoply™ Human PTPN11 Knockdown Stable Cell Line

Epidemiological surveys show that the incidence of type 2 diabetes mellitus (T2DM) and malignant tumors is rapidly rising globally, becoming one of the major diseases threatening human life. Here, researchers found that compared to non-diabetic patients, metabolic pathways such as oxidative phosphorylation, glycolysis/gluconeogenesis, and insulin secretion were significantly enriched in the tumor tissues of diabetic patients. Furthermore, researchers also identified the marker gene PTPN11, associated with T2DM and colorectal cancer (CRC), in diabetic tumor tissues. PTPN11 was highly expressed in diabetic tumor tissues compared to normal tissues. High PTPN11 expression predicts a poor prognosis in CRC. PTPN11 expression is closely related to immune-infiltrating cells in CRC. Gene set enrichment analysis (GSEA) showed that PTPN11 is enriched in cancer-related pathways. Western blotting analysis indicated that PTPN11 knockdown reduced the protein levels of p-PI3K, p-AKT, CDK1, and CYCLIN D, but did not alter the protein levels of PI3K and AKT. Cell proliferation and wound healing experiments showed that both PTPN11 and high glucose levels enhanced cell proliferation and migration. These findings suggest that PTPN11 may be a potential key biomarker for patients with diabetes and colorectal cancer, and will provide a new potential target for future treatment of T2DM complicated with CRC.

To determine the effects of PTPN11 and high glucose on CRC cells, researchers conducted scratch healing and cell proliferation experiments at the cellular level. Compared to NC cells, PTPN11 knockdown of SW480 cells significantly reduced migration ability under low glucose conditions. Furthermore, in PTPN11 knockdown cells, treatment with 11 mM glucose increased cell migration ability, but the difference was not significant. Moreover, under high glucose conditions, PTPN11 knockdown significantly weakened cell migration compared to NC cells (Figure 1A and 1B). Cell proliferation experiments showed that under low glucose conditions, the proliferation ability of PTPN11 knockdown SW480 cells was significantly reduced compared to NC cells. Treatment with 11 mM glucose significantly enhanced the proliferation ability of PTPN11 knockdown SW480 cells. Furthermore, under high glucose conditions, the proliferation ability of PTPN11 knockdown SW480 cells was significantly reduced compared to NC cells (Figure 1C). These results indicate that both high glucose and PTPN11 can promote the proliferation and migration of CRC cells.

Figure 1. Effect of high glucose and PTPN11 on migration and proliferation of SW480 cells. (Sun, Meiling, et al., 2024)