Cat.No. : CSC-SC011783 Host Cell: HEK293 (CHO and other cell types are also available)
| Cat. No. | CSC-SC011783 |
| Description | Using Creative Biogene's proprietary lentiviral vectors, we subclone the target gene into lentivector, generate the lentivirus particles, sequentially infect the cell line HEK293 (other cell types are also available according to your requirements), and select the clones constantly expressing target gene at high level. |
| Gene | PIK3CA |
| Gene Species | Homo sapiens (Human) |
| Host Cell | HEK293 (CHO and other cell types are also available) |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Size Form | 2 × 10^6 cells / vial |
| Shipping | Dry Ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Gene Name | PIK3CA phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit alpha [ Gallus gallus (chicken) ] |
| Gene Symbol | PIK3CA |
| Synonyms | PIK3CA; phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit alpha; C-P3K; phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha isoform; phosphoinositide 3-kinase catalytic subunit; phosphoinositide-3-kinase, catalytic, alpha polypeptide; |
| Gene Description | phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit alpha |
| Gene ID | 424971 |
| UniProt ID | O42391 |
| mRNA Refseq | NM_001004410 |
| Protein Refseq | NP_001004410 |
| Chromosome Location | chromosome: 9 |
| Pathway | Adrenergic signaling in cardiomyocytes; Apoptosis; EGFR1 Signaling Pathway; ErbB signaling pathway; Focal adhesion; FoxO signaling pathway; G13 Signaling Pathway; |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
Dihydrotanshinone I (DHTS), extracted from Salvia miltiorrhiza, has been previously identified as the most effective compound against cancer cells among Salvia miltiorrhiza extracts. However, the therapeutic benefits of DHTS in ovarian cancer and its underlying mechanisms remain unclear. Here, researchers confirmed that DHTS exhibits cytotoxic effects on chemotherapy-sensitive ovarian cancer cells, both alone and in combination with platinum-based chemotherapy. DHTS inhibited the proliferation and migration of ovarian cancer cells in vitro and in vivo by regulating the PI3K/AKT signaling pathway. Combination treatment with DHTS and cisplatin enhanced DNA damage in ovarian cancer cells. In summary, these findings suggest that DHTS induces ovarian cancer cell death by causing DNA damage and inhibits ovarian cancer cell proliferation and migration.
Here, researchers investigated whether DHTS inhibits ovarian cancer cell migration and invasion by regulating PIK3CA transcription. They performed wound healing assays and Transwell chamber assays on wild-type, empty vector-transfected, and PIK3CA-overexpressing (PIK3CA-OX) A2780 cells. In the wound healing assay, the wound closure rate of empty vector-transfected A2780 cells was similar to that of wild-type cells, while PIK3CA-overexpressing A2780 cells showed significantly higher migration ability after 24 hours of DHTS treatment compared to the two control cell lines (Figure 1A, B, upper panel). In the Transwell assay, cells were seeded into the upper chamber of the filter membrane and allowed to migrate for 24 hours in the presence of DHTS. The number of wild-type cancer cells that migrated to the lower side of the filter membrane was similar to that of empty vector-transfected cells. However, compared to the other two cell lines, the migration and invasion ability of PIK3CA-overexpressing A2780 cells was significantly enhanced by 30%-50% (Figure 1A, B, lower panel). These data suggest that PIK3CA is essential in the migration and invasion process of DHTS-treated ovarian cancer cells. On the other hand, DHTS may reduce the migratory ability of ovarian cancer cells by inhibiting the PI3K pathway.
Figure 1. Overexpression of PIK3CA gene increases cell migration and invasion of DHTS-treated A2780 ovarian cancer cells. (Wang X, et al., 2020)
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