Panoply™ Human NRG1 Over-expressing Stable Cell Line

Name: Panoply™ Human NRG1 Over-expressing Stable Cell Line
SKU: CSC-SC010619
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-SC010619

Host Cell : HEK293 (CHO and other cell types are also available) Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-SC010619
Description	Using Creative Biogene's proprietary lentiviral vectors, we subclone the target gene into lentivector, generate the lentivirus particles, sequentially infect the cell line HEK293 (other cell types are also available according to your requirements), and select the clones constantly expressing target gene at high level.
Target Gene	NRG1
Gene Species	Homo sapiens (Human)
Host Cell	HEK293 (CHO and other cell types are also available)
Host Cell Species	Species varies
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research
Size	2 × 10^6 cells / vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry Ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	NRG1 neuregulin 1 [ Homo sapiens ]
Gene Symbol	NRG1
Synonyms	GGF; HGL; HRG; NDF; ARIA; GGF2; HRG1; HRGA; SMDF; MST131
Gene Description	neuregulin 1
GeneID	3084
Uni ProtID	Q02297
mRNA Refseq	NM_013962.2
Protein Refseq	NP_039256.2
Chromosome Location	8p12
Function	ErbB-2 class receptor binding; ErbB-3 class receptor binding; ErbB-3 class receptor binding; cytokine activity; growth factor activity; growth factor activity; protein binding; protein tyrosine kinase activator activity; receptor binding; receptor tyrosine kinase binding; transcription cofactor activity; transmembrane receptor protein tyrosine kinase activator activity; transmembrane receptor protein tyrosine kinase activator activity;
Pathway	Adaptive Immune System, organism-specific biosystem; Constitutive PI3K/AKT Signaling in Cancer, organism-specific biosystem; DAP12 interactions, organism-specific biosystem; DAP12 signaling, organism-specific biosystem; Disease, organism-specific biosystem; Downregulation of ERBB2:ERBB3 signaling, organism-specific biosystem; Downstream Signaling Events Of B Cell Receptor (BCR), organism-specific biosystem;
MIM	142445

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Hepatocellular carcinoma (HCC) is the third leading cause of cancer-related deaths worldwide. Metastasis is a primary factor contributing to treatment failure; therefore, gaining a deeper understanding of the molecular mechanisms driving cancer cell invasion and migration is urgently needed. The Talin1 protein plays a pivotal role in mediating metastasis-associated invasion and migration. In this study, researchers found that both Talin1 mRNA and protein levels were significantly elevated in HCC tissues. Downregulation of Talin1 significantly inhibited HCC cell proliferation and metastasis both in vitro and in vivo. Furthermore, upregulation of NRG-1-a downstream target of Talin1-promoted HCC cell metastasis. Crucially, Talin1 downregulation suppressed HCC progression by reducing NRG1 mRNA stability, thereby modulating NRG1 expression and its role in mediating the PI3K/AKT signaling pathway. Collectively, these findings demonstrate that Talin1 influences cell proliferation, metastasis, and invasion by regulating the NRG1/PI3K/AKT axis, suggesting that Talin1 is a highly promising therapeutic target for HCC.

To elucidate the function of NRG-1 in hepatocellular carcinoma (HCC), researchers generated NRG-1- overexpressing HCC cells. Assays confirmed successful overexpression, as evidenced by elevated intracellular NRG-1 levels (Figure 1A). Concurrently, NRG-1 upregulation was observed to promote HCC cell proliferation (Figure 1B). Furthermore, NRG-1 overexpression enhanced the migration and invasion of HCC cells (Figure 1C, D). Subsequently, the researchers investigated the effect of Talin-1 regulation on NRG-1 expression. Knockdown of Talin-1 resulted in a significant reduction in NRG-1 expression levels (Figure 1E). Further analysis using quantitative PCR (qPCR) revealed that reduced Talin-1 levels led to a decrease in NRG-1 mRNA levels (Figure 1F). Notably, experiments utilizing the mRNA synthesis inhibitor actinomycin D (ActD) demonstrated that Talin-1 knockdown accelerated the degradation rate of NRG-1 mRNA (Figure 1G). Collectively, these findings indicate that NRG-1 plays a pivotal role in HCC progression and metastasis and is regulated by Talin-1.

Figure 1. NRG-1 upregulation induces HCC metastasis in vitro. (Liu J, et al., 2024)

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