Panoply™ Human MSTN Over-expressing Stable Cell Line

Name: Panoply™ Human MSTN Over-expressing Stable Cell Line
SKU: CSC-SC009843
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-SC009843

Host Cell : HEK293 (CHO and other cell types are also available) Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-SC009843
Description	Using Creative Biogene's proprietary lentiviral vectors, we subclone the target gene into lentivector, generate the lentivirus particles, sequentially infect the cell line HEK293 (other cell types are also available according to your requirements), and select the clones constantly expressing target gene at high level.
Target Gene	MSTN
Gene Species	Homo sapiens (Human)
Host Cell	HEK293 (CHO and other cell types are also available)
Host Cell Species	Species varies
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research
Size	2 × 10^6 cells / vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry Ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	MSTN myostatin [ Homo sapiens ]
Gene Symbol	GDF8
Synonyms	GDF8; MSLHP
Gene Description	growth differentiation factor 8
GeneID	17700
Uni ProtID	O14793
mRNA Refseq	NM_005259.2
Protein Refseq	NP_005250.1
Chromosome Location	2q32.2
Function	cytokine activity; growth factor activity; heparin binding; receptor binding; contributes_to receptor binding;
MIM	601788

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Abnormalities in paravertebral muscle (PVM) play a significant role in the pathogenesis of idiopathic scoliosis (IS); although elevated oxidative stress may contribute to PVM damage in IS patients, the specific mechanisms underlying this oxidative stress remain unclear. Here, researchers found that primary skeletal muscle mesenchymal progenitor cells (hSM-MPCs)-key cells in vertebrate skeletal muscle myogenesis-derived from IS patients exhibited increased apoptosis, impaired myogenesis, and elevated oxidative stress. RNA sequencing and subsequent analysis revealed a significant upregulation of myostatin (MSTN) expression in hSM-MPCs from IS patients. Overexpression of MSTN in control hSM-MPCs led to increased NADPH oxidase 4 (NOX4) expression, promoted reactive oxygen species (ROS) production and apoptosis, and inhibited myogenesis; conversely, knocking down MSTN in hSM-MPCs from IS patients reduced NOX4 expression, suppressed ROS production and apoptosis, and promoted myogenesis. Furthermore, MSTN overexpression in mouse PVM induced elevated oxidative stress and scoliosis without accompanying vertebral structural abnormalities. Collectively, these findings suggest that the abnormal changes in PVM and the accumulation of oxidative stress observed in IS patients may stem from MSTN upregulation, a process that likely contributes to the onset and progression of IS.

To verify the effects of MSTN upregulation, researchers constructed MSTN-overexpressing CT-hSM-MPCs (Figure 1A and 1B). As shown in Figure 1C, MSTN overexpression led to increased Bax protein levels and the Bax/Bcl2 ratio, but did not alter Bcl2 levels. Compared to control cells, the proportion of TUNEL-positive cells was significantly increased in MSTN-overexpressing CT-hSM-MPCs, while the proportion of EdU-positive cells was decreased (Figure 1D and 1E). Furthermore, MSTN-overexpressing CT-hSM-MPCs showed increased ROS production (Figure 1F). The results also showed that MYOG mRNA and protein levels were decreased in MSTN-overexpressing CT-hSM-MPCs, while NOX4 expression was significantly increased (Figure 1G and 1H). However, NOX2 protein levels were downregulated in MSTN-overexpressing CT-hSM-MPCs (Figure 1H).

Figure 1. MSTN overexpression in CT-hSM-MPCs increase ROS release and apoptosis. (Li J, et al., 2022)

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