Panoply™ Human MAP3K1 Over-expressing Stable Cell Line

Name: Panoply™ Human MAP3K1 Over-expressing Stable Cell Line
SKU: CSC-SC009165
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-SC009165

Host Cell : HEK293 (CHO and other cell types are also available) Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-SC009165
Description	Using Creative Biogene's proprietary lentiviral vectors, we subclone the target gene into lentivector, generate the lentivirus particles, sequentially infect the cell line HEK293 (other cell types are also available according to your requirements), and select the clones constantly expressing target gene at high level.
Target Gene	MAP3K1
Gene Species	Homo sapiens (Human)
Host Cell	HEK293 (CHO and other cell types are also available)
Host Cell Species	Species varies
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research
Size	2 × 10^6 cells / vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry Ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	MAP3K1 mitogen-activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase [ Homo sapiens ]
Gene Symbol	MAP3K1
Synonyms	MEKK; MEKK1; SRXY6; MEKK 1; MAPKKK1
Gene Description	mitogen-activated protein kinase kinase kinase 1
GeneID	4214
Uni ProtID	Q13233
mRNA Refseq	NM_005921.1
Protein Refseq	NP_005912.1
Chromosome Location	5q11.2
Function	ATP binding; JUN kinase binding; JUN kinase kinase activity; JUN kinase kinase kinase activity; MAP kinase kinase kinase activity; identical protein binding; mitogen-activated protein kinase binding; mitogen-activated protein kinase kinase binding; protein binding; protein kinase activity; protein kinase activity; protein kinase binding; sphingolipid binding; ubiquitin-protein ligase activity; zinc ion binding;
Pathway	Activated TLR4 signalling, organism-specific biosystem; Apoptosis, organism-specific biosystem; Apoptosis Modulation by HSP70, organism-specific biosystem; BCR signaling pathway, organism-specific biosystem; CD40/CD40L signaling, organism-specific biosystem; CDC42 signaling events, organism-specific biosystem; Caspase cascade in apoptosis, organism-specific biosystem;
MIM	600982

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