Panoply™ Human FGFR3 Over-expressing Stable Cell Line

Name: Panoply™ Human FGFR3 Over-expressing Stable Cell Line
SKU: CSC-SC005722
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-SC005722

Host Cell : HEK293 (CHO and other cell types are also available) Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

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Gene Information

Cat. No.	CSC-SC005722
Description	Using Creative Biogene's proprietary lentiviral vectors, we subclone the target gene into lentivector, generate the lentivirus particles, sequentially infect the cell line HEK293 (other cell types are also available according to your requirements), and select the clones constantly expressing target gene at high level.
Target Gene	FGFR3
Gene Species	Homo sapiens (Human)
Host Cell	HEK293 (CHO and other cell types are also available)
Host Cell Species	Species varies
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research
Size	2 × 10^6 cells / vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry Ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	FGFR3 fibroblast growth factor receptor 3 [ Homo sapiens ]
Gene Symbol	FGFR3
Synonyms	ACH; CEK2; JTK4; CD333; HSFGFR3EX
Gene Description	fibroblast growth factor receptor 3 (achondroplasia, thanatophoric dwarfism)
GeneID	2261
Uni ProtID	P22607
mRNA Refseq	NM_001163213.1
Protein Refseq	NP_001156685.1
Chromosome Location	4p16.3
Function	ATP binding; fibroblast growth factor binding; fibroblast growth factor binding; fibroblast growth factor-activated receptor activity; protein binding; protein tyrosine kinase activity;
Pathway	Adaptive Immune System, organism-specific biosystem; Bladder cancer, organism-specific biosystem; Bladder cancer, conserved biosystem; Constitutive PI3K/AKT Signaling in Cancer, organism-specific biosystem; DAP12 interactions, organism-specific biosystem; DAP12 signaling, organism-specific biosystem; Disease, organism-specific biosystem;
MIM	134934

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Overexpression of fibroblast growth factor receptor 3 (FGFR3) is associated with tumor progression in various cancers. The role of FGFR3 in melanoma remains unclear. Here, the study shows that FGFR3 mRNA expression levels are higher in melanoma tissues than in normal healthy tissues. FGFR3 expression in cutaneous malignant melanoma (CMM) tissues is positively correlated with Breslow thickness and lymph node metastasis. In A357 cells, knockdown of the FGFR3 gene reduces caspase 3 activity and apoptosis, while increasing caspase 3 activity and apoptosis rate. Conversely, overexpression of FGFR3 enhances caspase 3 activity, proliferation, invasion, and migration, but reduces caspase 3 activity and apoptosis rate. Furthermore, knockdown of FGFR3 upregulates E-cadherin expression, downregulates N-cadherin and vimentin expression, and reduces the phosphorylation levels of ERK, AKT, and EGFR. In the MCC xenograft mouse model, knockdown of FGFR3 reduced tumor growth and metastasis. Therefore, these studies indicate that FGFR3 is highly expressed in CMM tissues and is associated with increased Breslow thickness and lymph node metastasis. FGFR3 may promote melanoma growth, metastasis, and EMT behavior by influencing the phosphorylation levels of ERK, AKT, and EGFR.

In vitro experiments showed that FGFR3 knockdown significantly inhibited the proliferation of A357 cells. At the same time, FGFR3 overexpression significantly promoted the proliferation of these cells. The apoptosis rate of FGFR3 knockdown cells was significantly increased. In contrast, the apoptosis rate of FGFR3 overexpression cells was significantly decreased (Figure 1e-f). Caspase-3 activity was significantly increased in FGFR3 knockdown A357 cells, while it was significantly decreased in FGFR3 overexpression cells (Figure 1g-h). The number of colonies formed in FGFR3 knockdown A357 cells was significantly reduced compared to control cells (Figure 1a-b). However, the number of colonies formed in FGFR3 overexpression A357 cells was significantly increased compared to control cells (Figure 1c-d).

Figure 1. The colony formation ability and apoptosis rate of A357 cells. (Li L, et al., 2019)

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