Panoply™ Human ERCC4 Over-expressing Stable Cell Line

Name: Panoply™ Human ERCC4 Over-expressing Stable Cell Line
SKU: CSC-SC005036
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-SC005036

Host Cell : HEK293 (CHO and other cell types are also available) Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-SC005036
Description	Using Creative Biogene's proprietary lentiviral vectors, we subclone the target gene into lentivector, generate the lentivirus particles, sequentially infect the cell line HEK293 (other cell types are also available according to your requirements), and select the clones constantly expressing target gene at high level.
Target Gene	ERCC4
Gene Species	Homo sapiens (Human)
Host Cell	HEK293 (CHO and other cell types are also available)
Host Cell Species	Species varies
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research
Size	2 × 10^6 cells / vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry Ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	ERCC4 excision repair cross-complementing rodent repair deficiency, complementation group 4 [ Homo sapiens ]
Gene Symbol	ERCC4
Synonyms	XPF; RAD1; ERCC11
Gene Description	excision repair cross-complementing rodent repair deficiency, complementation group 4
Gene ID	2072
Uni Prot ID	Q92889
m RNA Refseq	NM_005236.2
Protein Refseq	NP_005227.1
Chromosome Location	16p13.12
Function	damaged DNA binding; endodeoxyribonuclease activity; contributes_to endodeoxyribonuclease activity; protein C-terminus binding; protein N-terminus binding; protein binding; single-stranded DNA binding; single-stranded DNA specific endodeoxyribonuclease activity; contributes_to structure-specific DNA binding;
Pathway	DNA Repair, organism-specific biosystem; Dual incision reaction in GG-NER, organism-specific biosystem; Dual incision reaction in TC-NER, organism-specific biosystem; Fanconi anemia pathway, organism-specific biosystem; Fanconi anemia pathway, conserved biosystem; Formation of incision complex in GG-NER, organism-specific biosystem; Formation of transcription-coupled NER (TC-NER) repair complex, organism-specific biosystem;
MIM	133520

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