Panoply™ Human CPB2 Over-expressing Stable Cell Line

Name: Panoply™ Human CPB2 Over-expressing Stable Cell Line
SKU: CSC-SC003561
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-SC003561

Host Cell : HEK293 (CHO and other cell types are also available) Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

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Gene Information

Cat. No.	CSC-SC003561
Description	Using Creative Biogene's proprietary lentiviral vectors, we subclone the target gene into lentivector, generate the lentivirus particles, sequentially infect the cell line HEK293 (other cell types are also available according to your requirements), and select the clones constantly expressing target gene at high level.
Target Gene	CPB2
Gene Species	Homo sapiens (Human)
Host Cell	HEK293 (CHO and other cell types are also available)
Host Cell Species	Species varies
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research
Size	2 × 10^6 cells / vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry Ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	CPB2 carboxypeptidase B2 (plasma) [ Homo sapiens ]
Gene Symbol	CPB2
Synonyms	CPU; PCPB; TAFI
Gene Description	carboxypeptidase B2 (plasma)
GeneID	1361
Uni ProtID	Q96IY4
mRNA Refseq	NM_001872.3
Protein Refseq	NP_001863.2
Chromosome Location	13q14.11
Function	metallocarboxypeptidase activity; zinc ion binding;
Pathway	Complement and Coagulation Cascades, organism-specific biosystem; Complement and coagulation cascades, organism-specific biosystem; Complement and coagulation cascades, conserved biosystem; Pancreatic secretion, organism-specific biosystem; Pancreatic secretion, conserved biosystem; Protein digestion and absorption, organism-specific biosystem; Protein digestion and absorption, conserved biosystem;
MIM	603101

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Hepatocellular carcinoma (HCC) is a type of liver cancer characterized by high incidence and extremely high mortality rates. Given the limitations of current therapies-such as high recurrence rates and drug resistance-there is an urgent need to explore innovative treatment strategies. In this study, researchers established lenvatinib-resistant Huh7-LR and PLC/PRF/5-LR cell lines, which exhibited significant resistance indices of 4.59 and 4.37, respectively. RNA sequencing analysis revealed 82 differentially expressed genes in HCC tissues associated with lenvatinib resistance. A prognostic model was developed to stratify patients into high-risk and low-risk groups, revealing a significant difference in overall survival between the two; a nomogram demonstrated good concordance in predicting survival probability. The study identified CPB2 as a key gene associated with lenvatinib resistance; low CPB2 expression in HCC tissues correlated with poor prognosis, and the gene was found to promote HCC progression and lenvatinib resistance by inhibiting the MAPK signaling pathway.

Previous analyses have confirmed that CPB2 is a protective prognostic factor (HR < 1) and a key gene associated with lenvatinib resistance. In this study, researchers analyzed the TCGA-LIHC dataset and compared CPB2 expression between hepatocellular carcinoma (HCC) tissues and adjacent non-tumor tissues; the results revealed low levels of both CPB2 mRNA and protein in tumor tissues (Figure 1a-c). Kaplan-Meier analysis and log-rank tests demonstrated that low CPB2 expression was positively correlated with shorter overall survival (OS; Figure 1d), progression-free interval (PFI; Figure 1e), and disease-specific survival (DSS; Figure 1f). These findings suggest that reduced CPB2 expression in HCC tissues promotes tumor progression. To validate this conclusion, the researchers generated CPB2-overexpressing HCC cell lines (Huh7 and PLC/PRF/5) and assessed the impact on cell proliferation using CCK-8 and EdU assays. Western blotting confirmed the overexpression (Figure 1g). The results showed that, compared with the control group, the proliferation of CPB2-overexpressing HCC cells was significantly inhibited (Figure 1h and i), and DNA replication capacity was reduced (Figure 1j). Furthermore, elevated CPB2 expression suppressed the migration and invasion capabilities of HCC cells (Figure 1k and l).

Figure 1. Low CPB2 expression in HCC is associated with poor prognosis and promotes tumor progression. (Liu K, et al., 2026)

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