Cat.No. : CSC-SC002753 Host Cell: HEK293 (CHO and other cell types are also available)
| Cat. No. | CSC-SC002753 |
| Description | Using Creative Biogene's proprietary lentiviral vectors, we subclone the target gene into lentivector, generate the lentivirus particles, sequentially infect the cell line HEK293 (other cell types are also available according to your requirements), and select the clones constantly expressing target gene at high level. |
| Gene | CD46 |
| Gene Species | Homo sapiens (Human) |
| Host Cell | HEK293 (CHO and other cell types are also available) |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Size Form | 2 × 10^6 cells / vial |
| Shipping | Dry Ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Gene Name | CD46 CD46 molecule, complement regulatory protein [ Homo sapiens ] |
| Gene Symbol | CD46 |
| Synonyms | CD46; CD46 molecule, complement regulatory protein; antigen identified by monoclonal TRA 2 10 , CD46 antigen, complement regulatory protein , MCP, membrane cofactor protein (CD46, trophoblast lymphocyte cross reactive antigen) , MIC10; membrane cofactor protein; MGC26544; TLX; TRA2.10; measles virus receptor; complement membrane cofactor protein; trophoblast leucocyte common antigen; trophoblast leukocyte common antigen; CD46 antigen, complement regulatory protein; trophoblast-lymphocyte cross-reactive antigen; antigen identified by monoclonal TRA-2-10; membrane cofactor protein (CD46, trophoblast-lymphocyte cross-reactive antigen); MCP; AHUS2; MIC10; |
| Gene ID | 4179 |
| UniProt ID | P15529 |
| mRNA Refseq | BC030594 |
| Chromosome Location | 1q32 |
| Pathway | Complement and Coagulation Cascades, organism-specific biosystem; Complement and coagulation cascades, organism-specific biosystem; Complement and coagulation cascades, conserved biosystem; Complement cascade, organism-specific biosystem; Immune System, organism-specific biosystem; Innate Immune System, organism-specific biosystem; Measles, organism-specific biosystem; |
| MIM | 120920 |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
Swine influenza, caused by swine influenza A virus (swIAV), is an acute respiratory viral illness. Although several host factors have been identified that influence swIAV replication, the role of CD46 in this process remains unclear. Here, researchers demonstrate that CD46 inhibits swIAV replication by promoting type I interferon (IFN) production in porcine kidney cells (PK-15). Viral infection of CD46-overexpressing PK-15 cells demonstrated inhibition of H1N1 and H3N2 influenza virus (swIAV) replication and enhanced production of type I IFNs (IFN-α, IFN-β), interferon regulatory factor (IRF) 3, and mitochondrial antiviral signaling protein (MAVS). Viral infection of CD46-knockout PK-15 cells exhibited the opposite effect. Further results showed that CD46 knockout PK-15 cells had better influenza virus proliferation capacity than MDCK cells and PK-15 cells. These results indicate that CD46 is a promising target for regulating swIAV replication and will help develop new drugs to prevent viral infection and replication.
To determine whether CD46 regulates IFN expression during swIAV infection, CD46-KO, CD46-overexpressing, and WT PK-15 cells were infected with swIAV, and IFN expression was assessed by qRT-PCR. H1N1 infection experiments in CD46-KO PK-15 cells showed decreased expression of IFN-α, IFN-β, and mitochondrial antiviral signaling protein (MAVS) compared with WT PK-15 cells; whereas in CD46-overexpressing cells, expression of IFN-α, IFN-β, IRF3, and MAVS was increased compared with WT PK-15 cells (Figure 1A). H3N2 infection experiments in CD46-KO PK-15 cells showed that the expression of IFN-α and IFN-β was reduced at 12 hours compared with WT PK-15 cells; infection experiments in CD46-overexpressing cells showed that the expression of IFN-α, IFN-β, IRF3, and MAVS was increased compared with WT PK-15 cells (Figure 1B), especially IFN-β expression in cells infected with H1N1 and H3N2 viruses. These results suggest that CD46 knockout cell lines may promote the replication ability of swIAV by inhibiting the expression of IFN-α, IFN-β, IRF3, and MAVS.
Figure 1. CD46 suppresses the expression of IFNs in swIAVs infection. (Li H, et al., 2024)
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