Panoply™ Human CD46 Over-expressing Stable Cell Line

Name: Panoply™ Human CD46 Over-expressing Stable Cell Line
SKU: CSC-SC002753
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-SC002753

Host Cell : HEK293 (CHO and other cell types are also available) Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-SC002753
Description	Using Creative Biogene's proprietary lentiviral vectors, we subclone the target gene into lentivector, generate the lentivirus particles, sequentially infect the cell line HEK293 (other cell types are also available according to your requirements), and select the clones constantly expressing target gene at high level.
Target Gene	CD46
Gene Species	Homo sapiens (Human)
Host Cell	HEK293 (CHO and other cell types are also available)
Host Cell Species	Species varies
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research
Size	2 × 10^6 cells / vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry Ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	CD46 CD46 molecule, complement regulatory protein [ Homo sapiens ]
Gene Symbol	CD46
Synonyms	CD46; CD46 molecule, complement regulatory protein; antigen identified by monoclonal TRA 2 10 , CD46 antigen, complement regulatory protein , MCP, membrane cofactor protein (CD46, trophoblast lymphocyte cross reactive antigen) , MIC10; membrane cofactor protein; MGC26544; TLX; TRA2.10; measles virus receptor; complement membrane cofactor protein; trophoblast leucocyte common antigen; trophoblast leukocyte common antigen; CD46 antigen, complement regulatory protein; trophoblast-lymphocyte cross-reactive antigen; antigen identified by monoclonal TRA-2-10; membrane cofactor protein (CD46, trophoblast-lymphocyte cross-reactive antigen); MCP; AHUS2; MIC10;
Gene ID	4179
Uni Prot ID	P15529
m RNA Refseq	BC030594
Chromosome Location	1q32
Pathway	Complement and Coagulation Cascades, organism-specific biosystem; Complement and coagulation cascades, organism-specific biosystem; Complement and coagulation cascades, conserved biosystem; Complement cascade, organism-specific biosystem; Immune System, organism-specific biosystem; Innate Immune System, organism-specific biosystem; Measles, organism-specific biosystem;
MIM	120920

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Swine influenza, caused by swine influenza A virus (swIAV), is an acute respiratory viral illness. Although several host factors have been identified that influence swIAV replication, the role of CD46 in this process remains unclear. Here, researchers demonstrate that CD46 inhibits swIAV replication by promoting type I interferon (IFN) production in porcine kidney cells (PK-15). Viral infection of CD46-overexpressing PK-15 cells demonstrated inhibition of H1N1 and H3N2 influenza virus (swIAV) replication and enhanced production of type I IFNs (IFN-α, IFN-β), interferon regulatory factor (IRF) 3, and mitochondrial antiviral signaling protein (MAVS). Viral infection of CD46-knockout PK-15 cells exhibited the opposite effect. Further results showed that CD46 knockout PK-15 cells had better influenza virus proliferation capacity than MDCK cells and PK-15 cells. These results indicate that CD46 is a promising target for regulating swIAV replication and will help develop new drugs to prevent viral infection and replication.

To determine whether CD46 regulates IFN expression during swIAV infection, CD46-KO, CD46-overexpressing, and WT PK-15 cells were infected with swIAV, and IFN expression was assessed by qRT-PCR. H1N1 infection experiments in CD46-KO PK-15 cells showed decreased expression of IFN-α, IFN-β, and mitochondrial antiviral signaling protein (MAVS) compared with WT PK-15 cells; whereas in CD46-overexpressing cells, expression of IFN-α, IFN-β, IRF3, and MAVS was increased compared with WT PK-15 cells (Figure 1A). H3N2 infection experiments in CD46-KO PK-15 cells showed that the expression of IFN-α and IFN-β was reduced at 12 hours compared with WT PK-15 cells; infection experiments in CD46-overexpressing cells showed that the expression of IFN-α, IFN-β, IRF3, and MAVS was increased compared with WT PK-15 cells (Figure 1B), especially IFN-β expression in cells infected with H1N1 and H3N2 viruses. These results suggest that CD46 knockout cell lines may promote the replication ability of swIAV by inhibiting the expression of IFN-α, IFN-β, IRF3, and MAVS.

Figure 1. CD46 suppresses the expression of IFNs in swIAVs infection. (Li H, et al., 2024)

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