Panoply™ Human ALOX12 Over-expressing Stable Cell Line

Name: Panoply™ Human ALOX12 Over-expressing Stable Cell Line
SKU: CSC-SC000521
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-SC000521

Host Cell : HEK293 (CHO and other cell types are also available) Size : >1x10⁶ frozen cells/vial

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Cell Line Information

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Gene Information

Cat. No.	CSC-SC000521
Description	Using Creative Biogene's proprietary lentiviral vectors, we subclone the target gene into lentivector, generate the lentivirus particles, sequentially infect the cell line HEK293 (other cell types are also available according to your requirements), and select the clones constantly expressing target gene at high level.
Target Gene	ALOX12
Gene Species	Homo sapiens (Human)
Host Cell	HEK293 (CHO and other cell types are also available)
Host Cell Species	Species varies
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research
Size	2 × 10^6 cells / vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry Ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	ALOX12 arachidonate 12-lipoxygenase [ Homo sapiens ]
Gene Symbol	ALOX12
Synonyms	LOG12; 12-LOX; 12S-LOX
Gene Description	arachidonate 12-lipoxygenase
Gene ID	239
Uni Prot ID	P18054
m RNA Refseq	NM_000697.2
Protein Refseq	NP_000688.2
Chromosome Location	17p13.1
Function	arachidonate 12-lipoxygenase activity; hepoxilin-epoxide hydrolase activity; iron ion binding; lipoxygenase activity; protein binding;
Pathway	Arachidonic acid metabolism, organism-specific biosystem; Arachidonic acid metabolism, conserved biosystem; Eicosanoid Synthesis, organism-specific biosystem; Serotonergic synapse, organism-specific biosystem;
MIM	152391

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Over the past few decades, numerous studies have demonstrated a close link between psychological stress and cancer. Furthermore, it has been reported that stress can induce gene mutations. Here, NGS results show that cancer patients who experienced stressful life events had higher levels of mutations in the FKBP5 and ALOX12 genes. Analysis of ALOX12 and FKBP5 gene expression in cancer patients, non-cancer patients, and various cancer cell lines revealed that ALOX12 and FKBP5 mRNA levels were downregulated only in cancer patients and cancer cell lines, but not in non-cancer controls. Re-overexpression of wild-type ALOX12 and FKBP5 significantly inhibited cell growth and invasion in cancer cell lines, as well as tumor growth in xenograft models. These findings suggest that stressful life experiences may induce cancer development by increasing somatic mutations in the ALOX12 and FKBP5 genes.

To assess the effects of ALOX12 and FKBP5 overexpression on cell death, researchers digested cells with trypsin, stained them with trypan blue, and then used a PWD C100-SE to automatically count the number of dead cells. In ALOX12- and FKBP5-overexpressing MCF-7 cells, the percentage of dead cells was 60% and 65%, respectively (Figure 1A). In ALOX12- and FKBP5-overexpressing FaDu cells, the percentages of dead cells were 92% and 63%, respectively (Figure 1B). They also used Matrigel transmembrane chambers to detect the potential inhibitory effects of WT-ALOX12 and WT-FKBP5 overexpression. Compared with sham-transfected control cells, overexpression of ALOX12 and FKBP5 in FaDu cells inhibited cell invasion by approximately 85%. Compared with mock-transfected cells, MFC-7 cells completely lost their invasive ability in ALOX12-overexpressing MCF-7 cells; while in FKBP5-overexpressing MCF-7 cells, cell invasion ability was inhibited by 90% (Figure 1C, D).

Figure 1. The effects of the recreated expression of ALOX12 and FKBP5 on the induction of cell death on MCF-7 cells (A) and FaDu cells (B) and the inhibition of the cellular invasion ability in MCF-7 (C) and FaDu (D) cell lines. (Kutluhan A, et al., 2024)

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