Human ALOX12 adenoviral particles

Name: Human ALOX12 adenoviral particles
SKU: AD00927Z
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : AD00927Z

Storage : -80℃ Shipping : Frozen on dry ice

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Virus Particles Information

Quality Control

Gene Information

Cat. No.	AD00927Z
Description	Adenovirus with ORF of arachidonate 12-lipoxygenase (ALOX12).
Gene	ALOX12
Species	Human
Titer	Varies lot by lot, for example, ≥1x10^10 IFU/mL, ≥1x10^11 IFU/mL, ≥1x10^11 VP/mL etc.
Size	Varies lot by lot, for example, 100 ul, 500 ul, 1 mL etc.
Storage	Store at -80℃. Avoid multiple freeze/thaw cycles.
Shipping	Frozen on dry ice

Summary	Creative Biogene ensures high-quality adenovirus particles by optimizing and standardizing production protocols and performing stringent quality control (QC). The specific QC experiments performed vary between adenovirus particle lots.
Endotoxin	Endotoxins, primarily derived from Gram-negative bacteria, can trigger adverse immune responses. Endotoxin contamination is a significant concern in adenovirus production, especially for applications in animal studies and gene therapy. Creative Biogene utilizes rigorous endotoxin detection methods to monitor the endotoxin level in our produced adenovirus particles to ensure regulatory compliance.
Sterility	Creative Biogene ensures that adenovirus products are free of any bacterial, fungal and other microbial contamination.
Ad5 E1 Detection	All Creative Biogene adenoviruses are PCR tested to ensure that there are no detectable E1 sequences in the particles, which could be from revertants or external E1 contamination.
RCA Assays	Adenovirus products originating at Creative Biogene are guaranteed to have undetectable replication-competent adenovirus (RCA). This quality control measure is important because there is always the possibility of wild-type contamination due to revertants or environmental sources.
PFU Titering	All purified adenovirus preparations are tested for infectious titer. Creative Biogene's PFU test takes a few days longer but counts true plaques in HEK cells rather than estimating PFU titers via IHC staining or TCI50 of infected cells.

Gene Name	ALOX12 arachidonate 12-lipoxygenase [ Homo sapiens ]
Gene Symbol	ALOX12
Synonyms	LOG12; 12-LOX; 12S-LOX
Gene Description	arachidonate 12-lipoxygenase
Gene ID	239
Uni Prot ID	P18054
m RNA Refseq	NM_000697.2
Protein Refseq	NP_000688.2
Chromosome Location	17p13.1
Function	arachidonate 12-lipoxygenase activity; hepoxilin-epoxide hydrolase activity; iron ion binding; lipoxygenase activity; protein binding;
Pathway	Arachidonic acid metabolism, organism-specific biosystem; Arachidonic acid metabolism, conserved biosystem; Eicosanoid Synthesis, organism-specific biosystem; Serotonergic synapse, organism-specific biosystem;
MIM	152391

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