Nos2 Knockout Mouse Bone Marrow Macrophages

Name: Nos2 Knockout Mouse Bone Marrow Macrophages
SKU: CSC-RK0146
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RK0146

Target Gene : Nos2 Host Cell : Bone marrow macrophages

Size : >1x10⁶ cells/vial Validation : Sequencing

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RK0146
Background	Nitric oxide synthase, inducible (iNOS) is an enzyme that in humans is encoded by the NOS2 gene. Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. This gene encodes a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. Three related pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17. Alternative splicing of this gene results in two transcript variants encoding different isoforms.
Target Gene	Nos2
Abbr	MPBMMACs-Nos2 Knockout
Alias	NOS2, MAC-NOS, iNOS, Nos-2, Nos2a, i-NOS, NOS-II
Host Cell	Bone marrow macrophages
Host Cell Species	Mus musculus (Mouse)
Size	>1x10⁶ cells/vial
Validation	Sequencing
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties	Adherent

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	Nos2 nitric oxide synthase 2, inducible [ Mus musculus ]
Gene Symbol	Nos2
Synonyms	iNOS; Nos-2; Nos2a; i-NOS; NOS-II
Gene Description	nitric oxide synthase 2, inducible, macrophage
Gene ID	18126
Uni Prot ID	P29477
m RNA Refseq	NM_010927.3
Protein Refseq	NP_035057.1
Chromosome Location	11 B5; 11 45.6 cM
Function	FMN binding; Hsp90 protein binding; NADP binding; arginine binding; cAMP-dependent protein kinase regulator activity; calmodulin binding; flavin adenine dinucleotide binding; heme binding; iron ion binding; metal ion binding; nitric-oxide synthase activity; nitric-oxide synthase activity; oxidoreductase activity; protein binding; protein homodimerization activity; protein homodimerization activity; receptor binding; tetrahydrobiopterin binding;
Pathway	Amoebiasis, organism-specific biosystem; Amoebiasis, conserved biosystem; Arginine and proline metabolism, organism-specific biosystem; Arginine and proline metabolism, conserved biosystem; Calcium signaling pathway, organism-specific biosystem; Calcium signaling pathway, conserved biosystem; Chagas disease (American trypanosomiasis), organism-specific biosystem;

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Q & A

Customer Reviews

Customer Q&As

How is the knockout cell line validated?

A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.

Is the product a single clonal cell or mixed cell pool?

A: Single clonal cell.

Can I confirm gene knockout by RT-qPCR?

A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.

How can I store the cell product?

A: The cell line should be stored in liquid nitrogen for long-term preservation.

Is it possible to get multiple knockout clones for my GOI?

A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.

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