Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : CLOE-2358 Host Cell : HEK293
| Cat. No. | CLOE-2358 |
| Introduction | This cell lysate over-expresses mouse S100b (P50114) (Ser2-Glu92) fused with human IgG1-Fc at the N-terminus. Purification of target protein (s) from this cell lysate has been verified. |
| Target Gene | S100b |
| Host Cell | HEK293 |
| Host Cell Species | Homo sapiens (Human) |
| Applications | Positive control for Western Blot (WB), antibody verification, protein purification |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Storage | Upon receipt, store at 4°C, or prepare aliquots and store at -80°C. Multiple freeze-thaw cycles should be avoided. |
| Shipping | Ice package |
| Gene Name | S100b S100 protein, beta polypeptide, neural [ Mus musculus ] |
| Gene Symbol | S100b |
| Synonyms | Bpb; AI850290 |
| Gene Description | S100 protein, beta polypeptide, neural |
| Gene ID | 20203 |
| Uni Prot ID | P50114 |
| m RNA Refseq | NM_009115.3 |
| Protein Refseq | NP_033141.1 |
| Chromosome Location | 10 C1; 10 41.0 cM |
| Pathway | Activated TLR4 signalling, organism-specific biosystem; Advanced glycosylation endproduct receptor signaling, organism-specific biosystem; Cytosolic sensors of pathogen-associated DNA, organism-specific biosystem; DAI mediated induction of type I IFNs, organism-specific biosystem; Immune System, organism-specific biosystem; Innate Immune System, organism-specific biosystem; MyD88 cascade initiated on plasma membrane, organism-specific biosystem; |
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