Human S100B HEK293 Cell Lysate

Name: Human S100B HEK293 Cell Lysate
SKU: CLOE-1648
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CLOE-1648 Host Cell : HEK293

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Cell Lysate Information

Gene Information

Cat. No.	CLOE-1648
Introduction	This cell lysate over-expresses the human S100B (NP_006263.1) (Ser 2-Glu 92) fused with the Fc region of human IgG1 at the N-terminus. Purification of target protein (s) from this cell lysate has been verified.
Target Gene	S100B
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Applications	Positive control for Western Blot (WB), antibody verification, protein purification
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO
Storage	Upon receipt, store at 4°C, or prepare aliquots and store at -80°C. Multiple freeze-thaw cycles should be avoided.
Shipping	Ice package

Gene Name	S100B S100 calcium binding protein B [ Homo sapiens ]
Gene Symbol	S100B
Synonyms	NEF; S100; S100-B; S100beta
Gene Description	S100 calcium binding protein B
Gene ID	6285
Uni Prot ID	P04271
m RNA Refseq	NM_006272.2
Protein Refseq	NP_006263.1
Chromosome Location	21q22.3
Pathway	Activated TLR4 signalling, organism-specific biosystem; Advanced glycosylation endproduct receptor signaling, organism-specific biosystem; Cytosolic sensors of pathogen-associated DNA, organism-specific biosystem; DAI mediated induction of type I IFNs, organism-specific biosystem; Immune System, organism-specific biosystem; Innate Immune System, organism-specific biosystem; MyD88 cascade initiated on plasma membrane, organism-specific biosystem;
MIM	176990

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