Transfected Stable Cell Lines
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Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : CLOE-2896 Host Cell : HEK293
| Cat. No. | CLOE-2896 |
| Introduction | This cell lysate overexpresses mouse Pgf with five amino acids (DDDDK) at the C-terminus. Purification of target protein from this cell lysate has been verified. |
| Target Gene | Pgf |
| Host Cell | HEK293 |
| Host Cell Species | Homo sapiens (Human) |
| Applications | Positive control for Western Blot (WB), antibody verification, protein purification. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Storage | Upon receipt, store at 4°C, or prepare aliquots and store at -80°C. Multiple freeze-thaw cycles should be avoided. |
| Shipping | Ice package |
| Gene Name | Pgf placental growth factor [ Mus musculus ] |
| Gene Symbol | Pgf |
| Synonyms | PIGF; Plgf; AI854365 |
| Gene Description | placental growth factor |
| Gene ID | 18654 |
| Uni Prot ID | P49764 |
| m RNA Refseq | NM_008827.2 |
| Protein Refseq | NP_032853.1 |
| Chromosome Location | 12 D; 12 39.0 cM |
| Function | growth factor activity; protein heterodimerization activity; protein homodimerization activity; |
| Pathway | Bladder cancer, organism-specific biosystem; Bladder cancer, conserved biosystem; Focal Adhesion, organism-specific biosystem; Focal adhesion, organism-specific biosystem; Focal adhesion, conserved biosystem; HIF-1 signaling pathway, organism-specific biosystem; PI3K-Akt signaling pathway, organism-specific biosystem; |
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