Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : CLOE-2457 Host Cell : HEK293
| Cat. No. | CLOE-2457 |
| Introduction | This cell lysate over-expresses mouse Mcam (Q8R2Y2-1) (Met 1-Val 563) fused with C-terminal his tag. Purification of target protein (s) from this cell lysate has been verified. |
| Target Gene | Mcam |
| Host Cell | HEK293 |
| Host Cell Species | Homo sapiens (Human) |
| Applications | Positive control for Western Blot (WB), antibody verification, protein purification |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Storage | Upon receipt, store at 4°C, or prepare aliquots and store at -80°C. Multiple freeze-thaw cycles should be avoided. |
| Shipping | Ice package |
| Gene Name | Mcam melanoma cell adhesion molecule [ Mus musculus ] |
| Gene Symbol | Mcam |
| Synonyms | CD146; CD149; Muc18; s-endo; AV025631; 1-gicerin; s-gicerin |
| Gene Description | melanoma cell adhesion molecule |
| GeneID | 84004 |
| Uni ProtID | Q8R2Y2 |
| mRNA Refseq | NM_023061.2 |
| Protein Refseq | NP_075548.2 |
| Chromosome Location | 9 A5.1; 9 |
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