Human MCAM(His) HEK293 Cell Lysate

Name: Human MCAM(His) HEK293 Cell Lysate
SKU: CLOE-1716
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CLOE-1716 Host Cell : HEK293

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Cell Lysate Information

Gene Information

Cat. No.	CLOE-1716
Introduction	This cell lysate over-expresses the extracellular domain of human MCAM (NP_006491.2) (Met 1-Gly 559) fused with a polyhistidine tag at the C-terminus. Purification of target protein (s) from this cell lysate has been verified.
Target Gene	MCAM
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Applications	Positive control for Western Blot (WB), antibody verification, protein purification
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO
Storage	Upon receipt, store at 4°C, or prepare aliquots and store at -80°C. Multiple freeze-thaw cycles should be avoided.
Shipping	Ice package

Gene Name	MCAM melanoma cell adhesion molecule [ Homo sapiens ]
Gene Symbol	MCAM
Synonyms	CD146; MUC18
Gene Description	melanoma cell adhesion molecule
GeneID	4162
Uni ProtID	P43121
mRNA Refseq	NM_006500.2
Protein Refseq	NP_006491.2
Chromosome Location	11q23.3
MIM	155735

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