Transfected Stable Cell Lines
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Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : CLOE-2751 Host Cell : HEK293
| Cat. No. | CLOE-2751 |
| Introduction | This cell lysate overexpresses mouse Agt (Met1-Val477) with a C-terminal polyhistidine tag. Purification of target protein from this cell lysate has been verified. |
| Target Gene | Agt |
| Host Cell | HEK293 |
| Host Cell Species | Homo sapiens (Human) |
| Applications | Positive control for Western Blot (WB), antibody verification, protein purification. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Storage | Upon receipt, store at 4°C, or prepare aliquots and store at -80°C. Multiple freeze-thaw cycles should be avoided. |
| Shipping | Ice package |
| Gene Name | Agt angiotensinogen (serpin peptidase inhibitor, clade A, member 8) [ Mus musculus ] |
| Gene Symbol | Agt |
| Synonyms | AngI; AngII; Aogen; AI265500; Serpina8 |
| Gene Description | angiotensinogen (serpin peptidase inhibitor, clade A, member 8) |
| Gene ID | 11606 |
| Uni Prot ID | Q3UTR7 |
| m RNA Refseq | NM_007428.3 |
| Protein Refseq | NP_031454.3 |
| Chromosome Location | 8 E2; 8 68.0 cM |
| Function | acetyltransferase activator activity; angiotensin receptor binding; hormone activity; type 1 angiotensin receptor binding; type 1 angiotensin receptor binding; type 2 angiotensin receptor binding; type 2 angiotensin receptor binding; |
| Pathway | ACE Inhibitor Pathway, organism-specific biosystem; Adipogenesis, organism-specific biosystem; Class A/1 (Rhodopsin-like receptors), organism-specific biosystem; Fatty acid, triacylglycerol, and ketone body metabolism, organism-specific biosystem; G alpha (i) signalling events, organism-specific biosystem; G alpha (q) signalling events, organism-specific biosystem; GPCR downstream signaling, organism-specific biosystem; |
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