Transfected Stable Cell Lines
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Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : CLOE-1066 Host Cell : HEK293
| Cat. No. | CLOE-1066 |
| Introduction | This cell lysate over-expresses C-terminal polyhistidine tagged human AGT (NP_000020.1). Purification of target protein (s) from this cell lysate has been verified. |
| Target Gene | AGT |
| Host Cell | HEK293 |
| Host Cell Species | Homo sapiens (Human) |
| Applications | Positive control for Western Blot (WB), antibody verification, protein purification |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Storage | Upon receipt, store at 4°C, or prepare aliquots and store at -80°C. Multiple freeze-thaw cycles should be avoided. |
| Shipping | Ice package |
| Gene Name | AGT angiotensinogen (serpin peptidase inhibitor, clade A, member 8) [ Homo sapiens ] |
| Gene Symbol | AGT |
| Synonyms | AGT; angiotensinogen (serpin peptidase inhibitor, clade A, member 8); SERPINA8; angiotensinogen; alpha 1 antiproteinase; antitrypsin; serpin A8; angiotensin I; angiotensin II; pre-angiotensinogen; alpha-1 antiproteinase, antitrypsin; serine (or cysteine) proteinase inhibitor; ANHU; |
| Gene ID | 183 |
| Uni Prot ID | P01019 |
| m RNA Refseq | BC011519 |
| Chromosome Location | 1q42.2 |
| Function | acetyltransferase activator activity; growth factor activity; hormone activity; hormone activity; serine-type endopeptidase inhibitor activity; type 1 angiotensin receptor binding; type 2 angiotensin receptor binding; |
| Pathway | ACE Inhibitor Pathway, organism-specific biosystem; Adipogenesis, organism-specific biosystem; Class A/1 (Rhodopsin-like receptors), organism-specific biosystem; Fatty acid, triacylglycerol, and ketone body metabolism, organism-specific biosystem; G alpha (i) signalling events, organism-specific biosystem; G alpha (q) signalling events, organism-specific biosystem; GPCR downstream signaling, organism-specific biosystem; |
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