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MIR515-2-pCMV-MIR

For research use only. Not intended for any clinical use.
Cat.No.
MIPH2259
Description
Expression plasmid for human microRNA MIR515-2. All miRNA clones were sequenced to ensure that the pre-mir sequences match reference sequence in miRBase. The flanking sequence of a miRNA clone may differ from the NCBI reference with respect to biological polymorphisms. This should not affect the function of the mature miRNA. Both mature and minor (star) miRNA can be over expressed from the construct.
Reporter
GFP
Size/Form
The plasmid is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5 and 3 vector primers in separate vials.
Species
Human
Vector Type
plasmid
miRNA Information
Precursor Accession No.
MI0003147
Precursor Name
hsa-mir-515-2
Precursors sequence
UCUCAUGCAGUCAUUCUCCAAAAGAAAGCACUUUC UGUUGUCUGAAAGCAGAGUGCCUUCUUUUGGAGCG UUACUGUUUGAGA
Precursors Length
83 bp
Gene Description
Homo sapiens miR-515-2 stem-loop
Gene Species
Human
Mature miRNA-1 Accession No.
MIMAT0002826
Mature miRNA-1 Name
hsa-miR-515-5p
Mature miRNA-1 sequence
UUCUCCAAAAGAAAGCACUUUCUG
Mature miRNA-2 Accession No.
MIMAT0002827
Mature miRNA-2 Name
hsa-miR-515-3p
Mature miRNA-2 sequence
GAGUGCCUUCUUUUGGAGCGUU

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