MIR27a-pCMV-MIR

Name: MIR27a-pCMV-MIR
SKU: MIPH2026

For research use only. Not intended for any clinical use.

Cat.No.

MIPH2026

Description

Expression plasmid for human microRNA MIR27a. All miRNA clones were sequenced to ensure that the pre-mir sequences match reference sequence in miRBase. The flanking sequence of a miRNA clone may differ from the NCBI reference with respect to biological polymorphisms. This should not affect the function of the mature miRNA. Both mature and minor (star) miRNA can be over expressed from the construct.

Reporter

GFP

Size/Form

The plasmid is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5 and 3 vector primers in separate vials.

Species

Human

Vector Type

plasmid

miRNA Information

Precursor Accession No.

MI0000085

Precursor Name

hsa-mir-27a

Precursors sequence

CUGAGGAGCAGGGCUUAGCUGCUUGUGAGCAGGGU CCACACCAAGUCGUGUUCACAGUGGCUAAGUUCCG CCCCCCAG

Precursors Length

78 bp

Gene Description

Homo sapiens miR-27a stem-loop

Gene Species

Human

Mature miRNA-1 Accession No.

MIMAT0004501

Mature miRNA-1 Name

hsa-miR-27a-5p

Mature miRNA-1 sequence

AGGGCUUAGCUGCUUGUGAGCA

Mature miRNA-2 Accession No.

MIMAT0000084

Mature miRNA-2 Name

hsa-miR-27a-3p

Mature miRNA-2 sequence

UUCACAGUGGCUAAGUUCCGC

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