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LA Two-Step RT-PCR

For research use only. Not intended for any clinical use.
Cat.No.
RTTZ-02
Description
This kit is a two step RT-PCR kit designed for long first strand cDNA synthesis, enabling longer and more accurate RT-PCR products. The reverse transcriptase offers robust reverse transcription of virtually any RNA template including GC-rich, low copy and even highly structured RNA templates.
Applications
•RT-PCR for complex and low copy templates
•Detection of gene expression
•Generation of cDNA products for cloning and sequencing
Features
•High specificity and sensitivity
•Long cDNA synthesis
Report Gene
Two tube reaction and long cDNA products
Sample Type
RNA
Selection Marker
RNA
Shipping
Dry ice
Storage
Store at -20°C

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Q & A

Customer Reviews

Customer Q&As
What is the principle of RT-PCR?

A: In RT-PCR, the RNA template is first converted into complementary DNA (cDNA) using reverse transcriptase (RT). This cDNA is then used as a template for exponential amplification using PCR.

What is two-step RT-PCR?

A: Two-step RT-PCR requires two separate reactions, starting with first-strand cDNA synthesis (RT) and then amplifying a portion of the resulting cDNA by PCR in a separate tube. Therefore, two-step RT-PCR can be used to detect multiple genes in a single RNA sample.

What are advantages of two-step RT-qPCR?

A: (1) High sensitivity and compatible with limited sample input; (2) Ability to optimize RT and qPCR steps separately; (3) Can generate cDNA stock solution.

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