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RTTZ-02

LA Two-Step RT-PCR

For research use only. Not intended for any clinical use.

Cat. No. :   RTTZ-02

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Product Information

Cat. No. RTTZ-02
Description This kit is a two step RT-PCR kit designed for long first strand cDNA synthesis, enabling longer and more accurate RT-PCR products. The reverse transcriptase offers robust reverse transcription of virtually any RNA template including GC-rich, low copy and even highly structured RNA templates.
Sample Type RNA
Report Gene Two tube reaction and long cDNA products
Features •High specificity and sensitivity
•Long cDNA synthesis
Applications •RT-PCR for complex and low copy templates
•Detection of gene expression
•Generation of cDNA products for cloning and sequencing
Selection Marker RNA
Storage Store at -20°C
Shipping Dry ice
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What is the principle of RT-PCR?

A: In RT-PCR, the RNA template is first converted into complementary DNA (cDNA) using reverse transcriptase (RT). This cDNA is then used as a template for exponential amplification using PCR.

What is two-step RT-PCR?

A: Two-step RT-PCR requires two separate reactions, starting with first-strand cDNA synthesis (RT) and then amplifying a portion of the resulting cDNA by PCR in a separate tube. Therefore, two-step RT-PCR can be used to detect multiple genes in a single RNA sample.

What are advantages of two-step RT-qPCR?

A: (1) High sensitivity and compatible with limited sample input; (2) Ability to optimize RT and qPCR steps separately; (3) Can generate cDNA stock solution.

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