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3' RACE

For research use only. Not intended for any clinical use.
Cat.No.
RTRZ-01
Description
RT-PCR is difficult to get the full length of a gene. RACE (Rapid Amplification of cDNA Ends) allows identification of full-length cDNA, including 5', internal, and 3' untranslated regions (UTRs). This kit is powerful to obtain the 3' untranslated region of a target gene when only a partial sequence of a transcribed gene is available. This kit contains almost all of the reagents for 3' RACE. PCR products can be used for sequencing and cloning.
Applications
•3'RACE
•Full-length cDNA sequence cloning
Report Gene
cDNA synthesis from the 3'-end of poly(A) RNA
Sample Type
RNA
Selection Marker
RNA
Shipping
Dry ice
Storage
Store at -20°C

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Q & A

Customer Reviews

Customer Q&As
What is 3' RACE?

A: 3' RACE (Rapid Amplification of cDNA Ends) is a laboratory technique used to identify and amplify the 3' end of a specific mRNA molecule.

What is the principle of 3' RACE?

A: This technique involves reverse transcription of the mRNA using a specific primer known as the gene-specific primer (GSP), which hybridizes to the 3' end of the mRNA. The reverse transcription process is followed by PCR amplification using a GSP primer and a nested primer.

What are the advantages of RACE-sequencing?

A: Compared with traditional RACE fragment characterization by molecular cloning followed by Sanger sequencing of several clones, high-throughput sequencing characterization of RACE fragments is highly time-efficient, more sensitive, less expensive, and technically feasible.

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