3' RACE

Name: 3' RACE
SKU: RTRZ-01
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : RTRZ-01

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Product Information

Cat. No.	RTRZ-01
Description	RT-PCR is difficult to get the full length of a gene. RACE (Rapid Amplification of cDNA Ends) allows identification of full-length cDNA, including 5', internal, and 3' untranslated regions (UTRs). This kit is powerful to obtain the 3' untranslated region of a target gene when only a partial sequence of a transcribed gene is available. This kit contains almost all of the reagents for 3' RACE. PCR products can be used for sequencing and cloning.
Sample Type	RNA
Report Gene	cDNA synthesis from the 3'-end of poly(A) RNA
Applications	•3'RACE •Full-length cDNA sequence cloning
Selection Marker	RNA
Storage	Store at -20°C
Shipping	Dry ice

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What is 3' RACE？

A: 3' RACE (Rapid Amplification of cDNA Ends) is a laboratory technique used to identify and amplify the 3' end of a specific mRNA molecule.

What is the principle of 3' RACE?

A: This technique involves reverse transcription of the mRNA using a specific primer known as the gene-specific primer (GSP), which hybridizes to the 3' end of the mRNA. The reverse transcription process is followed by PCR amplification using a GSP primer and a nested primer.

What are the advantages of RACE-sequencing?

A: Compared with traditional RACE fragment characterization by molecular cloning followed by Sanger sequencing of several clones, high-throughput sequencing characterization of RACE fragments is highly time-efficient, more sensitive, less expensive, and technically feasible.

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